液相色谱测定调味料、肉制品中双乙酸钠含量的研究  

Determination of sodium diacetate(SDA)in seasoning and meat products by liquid chromatography

作　　者：周佳 刘子溱 文树新 杨振东 李雁 朱书强 ZHOU Jia;LIU Zizhen;WEN Shuxin;YANG Zhendong;LI Yan;ZHU Shuqiang(Gansu Food Inspection and Research Institute,Lanzhou 730300;Gansu Engineering Research Center of Nutrition and Safety Evaluation of Geographical Indication Products,Lanzhou 730300;Gansu Province Product Quality Supervision and Inspection Research Institute,Lanzhou 730050)

机构地区：[1]甘肃省食品检验研究院,兰州730300 [2]甘肃省地理标志产品营养与安全评价工程研究中心,兰州730300 [3]甘肃省产品质量监督检验研究院,兰州730050

出　　处：《中国食品添加剂》2023年第2期276-282,共7页China Food Additives

基　　金：甘肃省市场监督管理局科技计划项目(SSCJG-SP201915);陇原青年创新创业人才项目(2022LQGR63);甘肃省知识产权计划项目(21ZSCQ072)。

摘　　要：建立高效液相色谱测定调味料、肉制品中双乙酸钠含量的方法。样品经过加酸蒸馏和不加酸蒸馏,采用高效液相色谱-二极管阵列检测器(LC-PDA)对双乙酸钠含量进行测定,外标-换算系数法定量。样品中含有外加的乙酸不会影响双乙酸钠中游离乙酸的含量,可采用换算系数法来折算样品中的双乙酸钠含量。该方法在0.04~0.50 mg/mL浓度范围内有良好的线性关系,相关系数R2为0.9994,加标回收率在93.66%至97.72%之间,相对标准偏差在1.90%至3.29%的范围内,方法最低检出限为0.011g/kg,定量限为0.038 g/kg。该方法具有较高的灵敏度、准确性和重现性,可用于肉制品、调味料中双乙酸钠含量的测定,为相关产品中双乙酸钠含量的监测提供了监测方法,填补相关产品检测方法的空白。A method for determination of sodium diacetate in seasoning and meat products by liquid chromatography-photo diode array(LC-PDA)was established. The samples were distilled with and without acid,The content of sodium diacetate was determined by HPLC and quantified by external standard-conversion factor. The content of free acetic acid in sodium diacetate was not affected by the addition of acetic acid in the sample,and the content of sodium diacetate in the sample could be converted by the conversion factor. The method had a good linear relationship in the concentration range of 0.04 ~ 0.50 mg/mL,the correlation coefficient R2 was 0.9994,the spiked recovery rate was 93.66% to 97.72%,and the relative standard deviation(RSD)was 1.90% to 3.29%. The detection limits of the method were 0.011 g/kg,the quantification limits of the method were 0.038 g/kg. The method has high sensitivity,accuracy and reproducibility,and can be used for the determination of sodium diacetate content in meat products and seasonings,which provides a method for monitoring sodium diacetate content in related products,and fills the gap in detecting methods of relevant products.

关 键 词：双乙酸钠 乙酸钠 液相色谱 调味料及肉制品 换算系数 

分 类 号：TS207.3[轻工技术与工程—食品科学]