重瓣铁线莲花色与花青素的关系  

The Relationship Between the Colors and Anthocyanins of Clematis Hybridas with Double Petals

作　　者：袁明霞[1] 宋微[1] 刘换换 朱骏 陈天森 丁益 Yuan Mingxia;Song Wei;Liu Huanhuan;Zhu Jun;Chen Tiansen;Ding Yi(Jiangsu Vocational College of Agriculture and Forestry,Jurong,212400;Jiangsu Jichuanglanhai Technology Co.Ltd.,Suzhou,215000)

机构地区：[1]江苏农林职业技术学院,句容212400 [2]苏州极创澜海科技有限公司,苏州215000

出　　处：《分子植物育种》2023年第3期941-949,共9页Molecular Plant Breeding

基　　金：江苏农林职业技术学院科技计划项目(2022kj36);江苏省种业振兴揭榜挂帅项目(JBGS[2021]092)共同资助。

摘　　要：为探明重瓣铁线莲花色与花青素的关系,本研究采用液质联用检测技术(HPLC-MS)对4个色系重瓣铁线莲中6种主要花青素进行了定量分析。结果表明,各色系重瓣铁线莲中均含有花青素,但花青素单体含量及总量差异显著,且不同色系中优势花青素单体不同。红色系重瓣铁线莲花青素中芍药素和矢车菊素含量明显高于紫色系和蓝色系,最高可达30.7倍和13.0倍,说明芍药素和矢车菊素可能与红色着色相关。紫色系重瓣铁线莲花青素中飞燕草素含量高于红色系,但低于蓝色系;蓝色系重瓣铁线莲花青素中飞燕草素含量最高,说明飞燕草素含量可能影响着花色从紫色到蓝色的变化。供试白色系重瓣铁线莲中只检出了少许矢车菊素,未检测到其他5种花青素,说明纯白色系重瓣铁线莲中可能不含花青素物质,检出的少许矢车菊素与供试材料萼瓣端部的微红色有关。此外,红色系重瓣铁线莲的6种花青素总量远高于其他色系,说明萼瓣呈色不同与花青素总量差异有很大关系。本研究的实施为探明铁线莲属萼瓣呈色机理奠定了基础,亦为其花色育种提供了理论依据。In order to find out the relationship between the colors and anthocyanins of Clematis hybridas(Clematis florida Thunb.)with double petals,6 major anthocyanins in Clematis hybridas with double petals of 4 color systems were quantitatively analyzed by HPLC-MS.The results showed that anthocyanins were detected in all Clematis hybridas,but the monomer content and total amount of anthocyanins were significantly different,and the dominant anthocyanin monomer in different color systems were different.The contents of peonidin and cyanidin in red Clematis hybridas were significantly higher than those in purple and blue Clematis hybridas,up to 30.7 and 13.0 times,indicating that peonidin and cyanidin might be related to red coloring.The content of delphinidin in purple Clematis hybridas was higher than that in red Clematis hybridas,but lower than that in blue Clematis hybridas,and the content of delphinidin in blue Clematis hybridas was the highest,indicating that delphinidin content might affect the change of flower color from purple to blue.Only a little cyanidin was detected in white Clematis hybridas,but no other five anthocyanins were detected,indicating that the pure white Clematis hybridas should be free of anthocyanins,and the little cyanidin detected might be related to the reddish color at the end of the calyx petals of the test material.In addition,the total amount of 6 anthocyanins in red Clematis hybridas was much higher than that in other color systems,indicating that the different colors of calyx petals was closely related to the total amount of anthocyanins.The implementation of this study has laid a foundation for exploring the coloration mechanism in calyx petals of Clematis hybridas,and also provided a theoretical basis for the breeding of flower color of Clematis hybridas.

关 键 词：铁线莲属 重瓣 花色 花青素 

分 类 号：S682.19[农业科学—观赏园艺]