E3泛素连接酶Cbl家族在子宫颈鳞状细胞癌中的表达及临床意义  

Expression and clinical significance of E3 ubiquitin ligase Cbl family in cervical squamous cell carcinoma

作　　者：张邢松 沈蓉 朱兴华 缪小兵 ZHANG Xingsong;SHEN Rong;ZHU Xinghua;MIAO Xiaobing(Department of Pathology,Affiliated Tumor Hospital of Nantong University,Nantong Tumor Hospital,Nantong 226361,China)

机构地区：[1]南通大学附属肿瘤医院(南通市肿瘤医院)病理科,江苏南通226361

出　　处：《南京医科大学学报（自然科学版）》2023年第1期34-39,共6页Journal of Nanjing Medical University(Natural Sciences)

基　　金：南通市卫健委面上项目(MB2020019)。

摘　　要：目的:探讨Casitas B细胞淋巴瘤(Casitas B-lineage lymphoma,Cbl)蛋白家族在子宫颈鳞状细胞癌(cervical squamous cell carcinoma,CSCC)中的表达及临床意义。方法:利用免疫组化法检测114例CSCC中c-Cbl、Cbl-b和Cbl-c的表达情况,分析c-Cbl、Cbl-b和Cbl-c表达水平与CSCC患者临床病理特征、无瘤生存期(disease free survival,DFS)和总生存期(overall survival,OS)的关系。结果:114例CSCC患者中,c-Cbl高表达87例,低表达27例;Cbl-b高表达69例,低表达45例;Cbl-c高表达55例,低表达59例。c-Cbl在癌组织和癌旁鳞状上皮组织化学评分(histochemistry score,H-score)分别为143.80±8.76、95.13±6.54,其在癌组织中的表达高于癌旁鳞状上皮(P<0.001);Cbl-b在癌组织和癌旁鳞状上皮H-score分别为83.95±5.28、93.54±3.91,其在癌和癌旁鳞状上皮中的表达无差异(P=0.146);Cbl-c在癌组织和癌旁鳞状上皮H-score分别为83.62±6.34、36.54±4.64,其在癌组织中的表达高于癌旁鳞状上皮(P<0.001)。c-Cbl在高~中分化以及最大径<4 cm肿瘤中表达较高(P均<0.05),但c-Cbl表达与患者年龄、脉管侵犯、浸润深度、淋巴结转移以及FIGO分期无关(P均>0.05)。Cbl-b在浸润深度>2/3纤维肌层患者中表达较高(P=0.045),但Cbl-b表达与患者年龄、脉管侵犯、肿瘤分化、肿瘤最大径、淋巴结转移以及FIGO分期无关(P均>0.05)。Cbl-c在低分化肿瘤中表达较高(P=0.012),但Cbl-c表达与患者年龄、脉管侵犯、浸润深度、肿瘤最大径、淋巴结转移以及FIGO分期无关(P均>0.05)。Kaplan-Meier生存分析显示:c-Cbl高表达患者比低表达患者的DFS和OS长(P均<0.05);Cbl-b以及Cbl-c表达与患者的DFS和OS均无关(P均>0.05)。Cox单因素和多因素分析显示:c-Cbl表达是影响DFS的独立预后因素(P=0.042);c-Cbl表达是影响OS的相关因素,但不是OS的独立预后因素(P=0.096)。结论:c-Cbl高表达CSCC患者比低表达患者的DFS和OS延长;c-Cbl表达是影响DFS的独立预后因素,但不是OS的Objective:To investigate the expression and clinical significance of Casitas B-lineage lymphoma(Cbl)protein family in cervical squamous cell carcinoma(CSCC).Methods:Immunohistochemistry was used to detect the expression of c-Cbl,Cbl-b,and Cbl-c in 114 cases of CSCC.The correlations between c-Cbl,Cbl-b,and Cbl-c expression and clinicopathological characteristics,disease free survival(DFS)and overall survival(OS)were analyzed.Results:Of the 114 CSCC patients,87 had high c-Cbl expression and 27 had low c-Cbl expression;69 had high Cbl-b expression and 45 had low Cbl-b expression;55 had high Cbl-c expression and 59 had low Cbl-c expression.The histochemistry score(H-score)of c-Cbl in CSCC tissues and the adjacent normal tissues was 143.80±8.76 and95.13±6.54,respectively.c-Cbl expression was increased in CSCC compared with the adjacent normal tissues(P<0.001).The H-score of Cbl-b in CSCC tissues and the adjacent normal tissues was 83.95±5.28 and 93.54±3.91,respectively.There was no significant difference in Cbl-b expression between CSCC tissues and the adjacent normal tissues(P=0.146).The H-score of Cbl-c in CSCC tissues and the adjacent normal tissues was 83.62±6.34 and 36.54±4.64,respectively.Cbl-c expression was increased in CSCC compared with the adjacent normal tissues(P<0.001).c-Cbl expression was higher in highly to moderately differentiated tumors and tumors with a maximum diameter less than 4 cm(both P<0.05),but it was not related to the patient’s age,vascular invasion,depth of invasion,lymph node metastasis and FIGO stage(all P>0.05).Cbl-b expression was higher in patients with invasion depth>2/3 of fibromuscular layer(P=0.045),but it was not related to patient’s age,vascular invasion,tumor differentiation,maximum tumor diameter,lymph node metastasis and FIGO stage(all P>0.05).Cbl-c expression was higher in poorly differentiated tumors(P=0.012),but it was not related to patient’s age,vascular invasion,depth of invasion,maximum tumor diameter,lymph node metastasis and FIGO stage(all P>0.05).Kaplan

关 键 词：子宫颈肿瘤 鳞状细胞癌 泛素连接酶 C-CBL CBL-B Cbl-c 

分 类 号：R737.33[医药卫生—肿瘤]