白鲜种子休眠解除过程中的转录组分析  

Transcriptome Analysis of Dormancy Release in Dictamnus dasycarpus Turcz.Seeds

作　　者：闫宗圣 王乾 王蕾 张兰兰 郑开颜 郑玉光 尚晓瑛 房慧勇 YAN Zongsheng;WANG Qian;WANG Lei;ZHANG Lanlan;ZHENG Kaiyan;ZHENG Yuguang;SHANG Xiaoying;FANG Huiyong(College of Pharmacy,Hebei University of Chinese Medicine,Shijiazhuang 050200,China;Traditional Chinese Medicine Processing Technology Innovation Center of Hebei Province,Shijiazhuang 050200,China;Beijing Yingkerui Pharmaceutical Tecnology Co.,Ltd,Beijing 102200,China)

机构地区：[1]河北中医学院药学院,石家庄050200 [2]河北省中药炮制技术创新中心,石家庄050200 [3]北京盈科瑞创新医药股份有限公司,北京102200

出　　处：《种子》2022年第12期35-40,共6页Seed

基　　金：河北省中医药管理局项目(2020131);全国中药资源普查项目(Z 135080000022);河北中医药管理局科研计划项目(2020126);河北省自然科学基金(H 2019423140)。

摘　　要：以解除休眠过程不同阶段的种子为材料,通过高通量测序技术对样品转录组进行测序分析。共获得135.98 Gb的原始数据,302453条Unigenes,注释到七大功能数据库(GO、KEGG、KOG、NR、TrEMBL、Pfam、Swiss-Prot),分别有139276(NR:46.05%)、128449(TrEMBL:42.47%)、86498(Swiss-Prot:28.60%)、90564(KOG:29.94%)、103481(KEGG:34.21%)、108540(GO:35.89%)以及90410(Pfam:29.89%)条Unigenes得到功能注释。通过GO分类和KEGG Pathway富集性分析,分别归于59个GO类别和145条代谢途径。同时,有6081个Unigenes被注释为转录因子。利用高通量测序技术获得白鲜转录组信息,有助于从分子水平对白鲜进行深入研究。The seeds in different stages of dormancy release process were taken as materials,and the transcriptome of the samples was sequenced and analyzed by high-throughput sequencing technology.135.98 Gb of original data were generated,302453 Unigenes were annotated to 7 major functional databases(GO,KEGG,KOG,NR,TrEMBL,Pfam and Swiss-prot).139276(NR:46.05%),128449(TrEMBL:42.47%),86498(Swiss prot:28.60%),90564(KOG:29.94%),103481(KEGG:34.21%),108540(GO:35.89%)and 90410(Pfam:29.89%)Unigenes were annotated respectively.By GO classification and KEGG pathway enrichment analysis,59 GO categories and 145 metabolic pathways were assigned.Simultaneously,6081 Unigenes were annotated as transcription factors.Using high-throughput sequencing technology to obtain the transcriptome information of white fresh food was helpful to further study white fresh food at the molecular level.

关 键 词：白鲜 休眠解除 转录组 功能注释 

分 类 号：S567.239[农业科学—中草药栽培]