九叶青花椒叶绿体基因组结构及系统进化分析  

Analysis of Complete Chloroplast Genome Sequences and Phylogenetic Evolution of Zanthoxylum armatum’Jiuyeqing’

作　　者：刘霞 孙冲 黄勤琴 谢润泸 刘浩文 陈泽雄[1] LIU Xia;SUN Chong;HUANG Qin-qin;XIE Run-lu;LIU Hao-wen;CHEN Ze-xiong(College of Landscape Architecture and Life Science,Chongqing University of Arts and Sciences,Chongqing 402160,China;Spice Crops Research Institute,College of Horticulture and Gardening,Yangtze University,Wuhan,434025,Hubei,China;College of Biology and Food Engineering,Chongqing Three Gorges University,Chongqing,404000,China)

机构地区：[1]重庆文理学院园林与生命科学学院,重庆402160 [2]长江大学园艺园林学院/香辛作物研究院,湖北武汉434025 [3]重庆三峡学院生物与食品工程学院,重庆404000

出　　处：《林业科学研究》2023年第1期100-108,共9页Forest Research

基　　金：国家自然科学基金项目(31901324);重庆市自然科学基金面上项目(cstc2019jcyj-msxmX0693);重庆市教委青年项目(KJQN202201337;KJQN202101320)。

摘　　要：[目的]揭示九叶青花椒叶绿体基因组的结构特征及其与其他物种的系统进化关系,为花椒属种质资源的鉴定、新品种的培育及品种间的遗传分析提供参考。[方法]使用改良CTAB法提取九叶青花椒叶片总DNA。利用BGISeq-500平台进行高通量测序,SPAdes v3.13.0软件组装叶绿体基因组,通过GeSeq注释九叶青花椒的全叶绿体基因组信息,对其叶绿体基因组序列的结构特征、重复序列、密码子偏好性及系统进化关系进行分析。[结果]九叶青花椒叶绿体基因组为典型的四分体组成结构,全长序列为158579 bp,编码133个基因;测到19个串联重复序列,49个长片段重复,70个简单重复序列;九叶青花椒叶绿体基因组中的蛋白编码基因共有26398个密码子(不包括终止密码子),在密码子第三位碱基上有较强的A/T碱基偏好性。[结论]本研究首次组装了九叶青花椒叶绿体基因组全序列,明确了花椒属为单系类群,九叶青花椒与野花椒(Z.simulans)关系密切,这将为花椒的遗传信息、花椒种质资源评价、分子育种、cpSSR分子标记的开发及遗传多样性等研究提供了重要参考。[Objective]To reveal the structural characteristics and phylogenetic relationships of the chloroplast genomes of Zanthoxylum armatum’Jiuyeqing’,and provide reference for identifying germplasm resources,breeding new varieties and the genetic analysis among varieties of the genus Zanthoxylum.[Method]The total DNA of Z.armatum’Jiuyeqing’was extracted using a modified CTAB method.Highthroughput sequencing was performed using the BGISeq-500 platform,the chloroplast genome was assembled by SPAdes v3.13.0 software,the whole chloroplast genome information of Z.armatum’Jiuyeqing’was annotated by GeSeq software,and the structural characteristics,repetitive sequences,codon preference and phylogenetic relationship were analyzed.[Result]Z.armatum’Jiuyeqing’was a typical tetrad structure with a full-length sequence of 158579 bp,encoding 133 genes.19 tandem repeats were detected,49 long repeat sequences,70 simple sequence repeat(SSR)were detected.A total of 26398 codons(excluding stop codons)were detected in the protein-coding gene of the chloroplast genome of Z.armatum’Jiuyeqing’,and there was a strong A/T base preference at the third base of the codon.[Conclusion]The complete chloroplast genome sequences of Z.armatum’Jiuyeqing’was firstly assembled for the study.The results of phylogenetic analysis show that the genus Zanthoxylum is a monophyletic group,and the Z.armatum’Jiuyeqing’is closely related to Z.simulans.The results of the study enrich the genetic information of Zanthoxylum and improve important references for Zanthoxylum germplasm resource evaluation,molecular breeding,development of cpSSR molecular markers,and genetic diversity research.

关 键 词：高通量测序 花椒属 叶绿体基因组 九叶青花椒 系统进化 

分 类 号：S718.46[农业科学—林学]