高效液相色谱测定焙烤食品中6种甜味剂  

Determination of 6 kinds of sweeteners in bakery products by HPLC

作　　者：杜瑞 万丽斌 郭碧珊 李娜 高火亮 Du Rui;Wan Libin;Guo Bishan;Li Na;Gao Huoliang(He’nan Commerce Science Institute Co.,Ltd.,Zhengzhou 450002,China)

机构地区：[1]河南省商业科学研究所有限责任公司,郑州450002

出　　处：《分析仪器》2023年第1期58-63,共6页Analytical Instrumentation

基　　金：河南省科学院基本科研业务费项目(220611002)。

摘　　要：建立了高效液相色谱测定烘烤食品中6种甜味剂的分析方法。样品用5%甲醇水溶液提取离心,采用二极管检测器测定安赛蜜、糖精钠、阿斯巴甜、纽甜、甜菊糖苷,蒸发光检测器测定三氯蔗糖。除三氯蔗糖外,其他5种甜味剂在1~50μg/mL的浓度范围内呈线性关系,三氯蔗糖在10~500μg/mL范围内呈幂函数关系;6种甜味剂相关系数均大于0.999;平均回收率为80.3%~99.8%,相对标准偏差为0.6%~5.8%。该方法前处理简单,准确度高,重现性好,适用于焙烤食品中6种甜味剂的测定。The sample was extracted with 5%methanol in water and centrifuged.Acesulfame,sodium saccharin,aspartame,neotame and sativoside were determined with diode array detector.Sucralose was determined by diode array detector.Sucralose was detected by evaporative light scattering detector(ELSD).Except for sucralose,the other 5 sweeteners showed a linear relationship in the concentration range of 1—50μg/mL.Sucralose showed a power function in the concentration range of 10—500μg/mL.The correlation coefficients of the six sweeteners were all greater than 0.999.The average recoveries ranged from 80.3%to 99.8%.The relative standard deviations ranged from 0.6%to 5.8%.The method has simple pretreatment,high accuracy and good reproducibility.

关 键 词：焙烤食品 高效液相色谱 甜味剂 二极管阵列检测器 蒸发光检测器 

分 类 号：O657.72[理学—分析化学] TS207.3[理学—化学]