茶园扁刺蛾生防菌筛选、鉴定及其发酵条件优化  

Identification and Culture Optimization of Effective Biocontrol Agent on Thosea senensis for Tea Plantations

作　　者：贺望兴[1,2] 谢小群 杨普香[1,2] 李文金 李延升[1,2] 江新凤 毛平生[1,2] HE Wangxing;XIE Xiaoqun;YANG Puxiang;LI Wenjin;LI Yansheng;JIANG Xinfeng;MAO Pingsheng(Jiangxi Sericulture and Tea Research Institute,Nanchang,Jiangxi 330203,China;Industrial Crops Institute of Jiangxi,Nanchang,Jiangxi 330203,China)

机构地区：[1]江西省蚕桑茶叶研究所,江西南昌330203 [2]江西省经济作物研究所,江西南昌330203

出　　处：《福建农业学报》2022年第11期1463-1469,共7页Fujian Journal of Agricultural Sciences

基　　金：江西省重点研发项目(20201BBF61006);江西省茶叶质量与安全控制重点实验室项目(20192BCD40007);江西省星火计划项目(20161BBF61011)。

摘　　要：【目的】筛选适宜生态有机茶园防治扁刺蛾的生防菌。【方法】采用温度筛选法从自然罹病死亡的扁刺蛾肠道体内初筛生防菌,浸液饲喂法测定生防菌对扁刺蛾幼虫的室内毒力;综合菌株的形态特征、分子生物学鉴定以及构建的系统发育树分析进行菌株的鉴定;通过单因素和正交试验对生防菌的发酵参数条件进行优化。【结果】筛选到1株对扁刺蛾幼虫具有较高防效的内生生防菌X-11,扁刺蛾3龄幼虫取食经2.56×10^(8)个·mL^(-1)X-11菌液处理的茶枝条7 d后扁刺蛾累计校正死亡率达到90.5%,致死中时(LT_(50))仅为2.88 d。菌株X-11经16s rDNA分子生物学鉴定以及构建的系统发育树分析确定为偶氮还原拟杆菌(Paenibacillus azoreducens)。发酵条件优化试验表明,菌株X-11的适宜发酵条件为:菌株接种量2%,在发酵温度36℃条件下发酵培养60 h,菌株含量最高达1.34×10^(9)个·mL^(-1)。【结论】筛选到1株对扁刺蛾幼虫具有较高防效的内生生防菌x-11,可作为防治扁刺蛾幼虫的生物防治菌剂使用。【Objective】 A biocontrol microbe on Thosea senensis Walker suitable for organic tea agriculture was identified and optimal culture conditions studied. 【Method】 From the intestines of T. senensis died from disease in nature, microbes were cultured and screened by means of varying the temperature. Indoor virulence of the selected microbes on T. senensis was determined by immersion feeding and further identified by morphological, molecular biological, and phylogenetic tree analyses. Culture of the isolated strain was optimized by univariate and orthogonal experiments. 【Result】 The endophytic strain X-11 with high efficacy in controlling the pest was isolated. The 3^(rd) instar larvae of T. senensis fed with a 2.56×10^(8) conidia·mL^(-) X-11 solution displayed a cumulative corrected mortality rate of 90.5% with a median lethal time LT_(50) of 2.88 d on the 7^(th) day after treatment. Based on the 16S rDNA molecular identification and phylogenetic analysis, X-11 was determined to be Paenibacillus azoreducens. The optimized culture conditions for the bacterium included the use of 2% inoculum to culture for 60 h at 36℃ with a final count approaching 1.34×10^(9) conidia·mL^(-1).【Conclusion】 The endophytic strain with high control efficacy on T. senensis larvae was identified as P. azoreducens. It could be cultured to be used as a bioagent for the pest control at tea plantations.

关 键 词：扁刺蛾 菌株筛选 拟杆菌 发酵条件 正交试验 

分 类 号：S435[农业科学—农业昆虫与害虫防治]