手性毛细管电泳实验中手性选择剂取代度的影响研究  

Study on the Influence of the Substitution Degree of Hydroxypropyl-β-Cyclodextrin in Chiral Capillary Electrophoresis Experiments

作　　者：谢天尧[1] 邓爱玲 肖华 朱芳[1] Tianyao Xie;Ailing Deng;Hua Xiao;Fang Zhu(School of Chemistry,Sun Yat-Sen University,Guangzhou 510275,China)

机构地区：[1]中山大学化学学院,广州510275

出　　处：《大学化学》2023年第1期187-191,共5页University Chemistry

基　　金：中山大学实验教学研究(改革)项目。

摘　　要：针对手性毛细管电泳实验“手性药物氧氟沙星对映体的分离检测”的教学过程中,发现手性选择剂取代度对实验结果具有重要影响,详细研究了一系列不同取代度的羟丙基-β-环糊精对氧氟沙星对映体分离效果的影响。研究结果表明:对映体的分离度随取代度变化显著,且取代度DS=7.9时分离度最佳。依据取代度的测定方法,对市售羟丙基-β-环糊精的质量进行评估,以确保手性毛细管电泳实验结果的稳定性和良好的教学效果。Because of the important influence of the chiral selector substitution degree on the enantioseparation of ofloxacin in chiral capillary electrophoresis experiments,the effects of the substitution degree of hydroxypropyl-β-cyclodextrin(HP-β-CD)on the inclusion complex between ofloxacin and HP-β-CD were investigated according to different substitution degrees.The results showed that the enantioseparation resolution changed significantly with the substitution degree.Moreover,the best separation resolution was found for a DS(degree of substitution)=7.9.The methods of determining DS were also discussed.Using these methods,the quality of commercial hydroxypropyl-β-cyclodextrin products can be effectively monitored,and the stability and effectiveness in teaching of the chiral capillary electrophoresis experiment can be ensured.

关 键 词：手性毛细管电泳实验 取代度 手性药物分析 羟丙基-Β-环糊精 氧氟沙星 仪器分析实验 

分 类 号：G64[文化科学—高等教育学] O657[文化科学—教育学]