体外和体内评价法分析苦杏仁油抗氧化能力  

Antioxidant Capacities Analysis of Bitter Almond Oil via in vitro and in vivo Evaluation Methods

作　　者：韩金承 孟鑫 吴慎威 闫伊狄 陈瑞琦 HAN Jin-cheng;MENG Xin;WU Shen-wei;YAN Yi-di;CHEN Rui-qi(College of Food and Health,Jinzhou Medical University,Jinzhou 121000,China)

机构地区：[1]锦州医科大学食品与健康学院,辽宁锦州121000

出　　处：《保鲜与加工》2023年第2期31-36,共6页Storage and Process

基　　金：中国科学院生物基材料重点实验室开放基金课题(BMF-2020-05)。

摘　　要：通过体外和体内评价法分析苦杏仁油的抗氧化能力。以VC为对照进行体外抗氧化试验,测定苦杏仁油对1,1-二苯基-2-三硝基苯肼自由基(DPPH·)、超氧阴离子自由基(O_(2)^(-)·)、羟自由基(·OH)的清除能力及对Fe3+的还原能力;以VE为对照进行体内抗氧化试验,将5组小鼠分别给予不同浓度的苦杏仁油,连续灌胃4周后灌注50%乙醇造成氧化损伤,6 h后摘眼取血测定血清中超氧化物歧化酶(SOD)活性和丙二醛(MDA)含量。经过体外抗氧化试验证明,苦杏仁油对DPPH·、O_(2)^(-)·、·OH具有一定的清除能力,半数清除率(IC_(50))分别为0.215、0.378、0.679 mg/mL,对Fe^(3+)具有一定的还原能力;体内抗氧化试验显示,苦杏仁油可以提高小鼠血清中SOD活性并降低MDA含量,其中以0.15 mL/g mb剂量效果最佳。该研究结果可为苦杏仁油在抗氧化方面的开发奠定理论基础。The antioxidant capacities of bitter almond oil were evaluated in vitro and in vivo.In this study,vitamin C was used as control for antioxidant tests in vitro,and the scavenging abilities of bitter almond oil on DPPH radical,superoxide anion radical(O_(2)^(-)·),hydroxyl radical(·OH)and the reducing ability of Fe3+were determined.Subsequently,Vitamin E was used as the control group for in vivo antioxidant evaluation.Five groups of mice were given with different concentrations of bitter almond oil,respectively.After 4 weeks of continuous gavage,50% ethanol solution was perfused to cause oxidative damage.After 6 h,the superoxide dismutase(SOD)activity and malondialdehyde(MDA)content in sera were measured.The antioxidant tests in vitro showed that bitter almond oil presented certain scavenging abilities on DPPH·,O_(2)^(-)·,·OH,and the half scavenging rates dosages(IC_(50))were 0.215 mg/mL,0.378 mg/mL,0.679 mg/mL,respectively,which also had a certain reduction ability on Fe3+.The in vivo antioxidant test showed that bitter almond oil could increase SOD activity and reduce MDA content in mice sera,and the dosage of 0.15 mL/g mbhad the best effects.The results of this study could provide a theoretical foundation for the antioxidant development of bitter almond oil.

关 键 词：苦杏仁油 体外抗氧化 体内抗氧化 自由基 

分 类 号：TS255.1[轻工技术与工程—农产品加工及贮藏工程]