鉴定鸭血及掺假品种的多重PCR方法的建立及应用  

Establishment and Application of Multiple PCR Methods for Identifying Duck Blood and Adulterated Breeds

作　　者：于文莹 迟凯月 徐宁 马秋贺 马玉贺 高丽君 艾金霞 夏薇 YU Wenying;CHI Kaiyue;XU Ning;MA Qiuhe;MA Yuhe;GAO Lijun;AI Jinxia;XIA Wei(College of Medical Technology,Beihua University,Jilin 132013,China)

机构地区：[1]北华大学医学技术学院,吉林吉林132013

出　　处：《食品科技》2022年第12期307-312,共6页Food Science and Technology

基　　金：吉林省科技厅重点科技成果转化项目(20170307001YY);吉林省科技发展计划项目(20200404152YY,20200403047SF);北华大学研究生创新计划项目([2021]060号)。

摘　　要：目的:建立多重PCR方法,鉴别鸭血的真伪,同时鉴定猪血、羊血、牛血、鸡血4种常见掺假动物血。方法:采用试剂盒法和高效提取禽类血液DNA法提取鸭血及其伪品的基因组DNA,利用鸭血细胞色素b基因(Cytb)设计特异性引物,通过文献筛选掺假动物血液的特异性引物,优化五重PCR的反应体系及反应条件,进行方法学验证和市售鸭血样品的检测。结果:所建立多重PCR体系特异性强,引物间无交叉干扰,重复性好,检测灵敏度为1 ng/μL,对市售的9份鸭血进行抽样检测,结果表明本地鸭血市场确实存在掺假现象。结论:多重PCR体系的建立,可以准确、快速检测鸭血制品中掺假的动物血液,为鸭血制品真伪鉴定提供了新的方法。Objective:To establish a multi-PCR method to identify the authenticity of duck blood,and to identify four common adulterated animal bloods:pig blood,sheep blood,cattle blood and chicken blood.Methods:The genomic DNA of duck blood and its counterfeits was extracted by the kit method and the efficient extraction of poultry blood DNA,the specific primers of the duck blood cytochrome b gene(Cytb)were used,the specific primers of adulterated animal blood were screened through literature,the reaction system and reaction conditions of the five-fold PCR were optimized,and the methodological verification and detection of commercially available duck blood samples were carried out.Results:The results of this research,the multi-PCR system was highly specific,there was no cross-interference between primers,the reproducibility was good,the detection sensitivity was 1 ng/μL,and 9 parts of duck blood sold in the market were sampled,and the results showed that there was indeed adulteration in the local duck blood market.Conclusion:Through the establishment of multiple PCR systems,it can accurately and quickly detect adulterated animal blood in duck blood products,which provides a new method for the authenticity identification of duck blood products.

关 键 词：鸭血 动物血 基因组 多重PCR 分子鉴定 

分 类 号：TS207.3[轻工技术与工程—食品科学]