Chiral polypeptide nanoparticles as nanoadjuvants of nanovaccines for efficient cancer prevention and therapy  

作　　者：Yuanzhen Su Weiguo Xu Qi Wei Yang Ma Jianxun Ding Xuesi Chen 

机构地区：[1]School of Applied Chemistry and Engineering,University of Science and Technology of China,Hefei 230026,China [2]Key Laboratory of Polymer Ecomaterials,Changchun Institute of Applied Chemistry,Chinese Academy of Sciences,Changchun 130022,China [3]Department of Thoracic Surgery,China-Japan Union Hospital of Jilin University,Changchun 130033,China

出　　处：《Science Bulletin》2023年第3期284-294,M0004,共12页科学通报（英文版）

基　　金：supported by the National Key Research and Development Program(2022YFC2603500,2022YFC2603501,2021YFC2400600,2021YFC2400603,and 2021YFC2400604);the National Natural Science Foundation of China(52273158,52273159,U21A2099,52022095,and 52073280);the Science and Technology Development Program of Jilin Province(20210509005RQ,20210504001GH,20200404182YY,and 20200201322JC);the Special Project for City-Academy Scientific and Technological Innovation Cooperation of Changchun(21SH14);the Youth Innovation Promotion Association of Chinese Academy of Sciences(2019230).

摘　　要：The chirality of bioactive molecules is closely related to their functions.D-amino acids commonly distributed in the bacterial cell walls trigger a robust anti-infective immune response.Inspired by that,two kinds of chiral polypeptides,poly(L-phenylalanine)-block-poly(L-lysine)(PL-K)and poly(Lphenylalanine)-block-poly(D-lysine)(PD-K),were synthesized and used as nanoadjuvants of nanovaccines for cancer prevention and therapy.The amphiphilic polypeptides self-assembled into nanoparticles with a diameter of about 30 nm during ultrasonic-assisted dissolution in phosphate-buffered saline.The nanovaccines PL-K-OVA and PD-K-OVA were easily prepared by mixing solutions of PL-K or PD-K and the model antigen chicken ovalbumin(OVA),respectively,with loading efficiencies of almost 100%.Compared to PL-K-OVA,PD-K-OVA more robustly induced dendritic cell maturation,antigen cross-presentation,and adaptive immune response.More importantly,it effectively prevented and treated the OVA-expressed B16-OVA melanoma model.PD-K-OVA achieved a tumor inhibition rate of 94.9%and even 97.0%by combining with anti-PD-1 antibody.Therefore,the chiral polypeptide nanoparticles represent simple,efficient,and extensively applicable nanoadjuvants for various nanovaccines.

关 键 词：Chiral polypeptide Nanoadjuvant NANOVACCINE Immunoactivation Cancer prevention and treatment 

分 类 号：R730.5[医药卫生—肿瘤]