多功能木聚糖水解酶的研究及其在食品领域的潜在应用  

Research of Multifunctional Xylanolytic Enzyme and Its Potential Applications in the Food Field

作　　者：韩雪 王钰璐 于海燕 辛凤姣[1] HAN Xue;WANG Yulu;YU Haiyan;XIN Fengjiao(Institute of Food Science and Technology,Chinese Academy of Agricultural Sciences,Beijing 100193,China)

机构地区：[1]中国农业科学院农产品加工研究所,北京100193

出　　处：《食品与生物技术学报》2023年第1期40-54,共15页Journal of Food Science and Biotechnology

基　　金：中国农业科学院农业科技创新工程项目(CAAS-ASTIP-2021-IFST-SN2021-07);中央级公益性科研院所基本科研业务费专项(S2020JBKY-13)。

摘　　要：木聚糖是植物细胞壁中含量最丰富的异质多糖,具有合成高值化生物基产品的巨大潜力。在食品领域,木聚糖经高效酶解转化可产生低聚木糖、木糖醇、阿魏酸等功能性食品多糖。与多酶协同催化相比,兼具多种木聚糖水解酶活性的多功能酶具有生产成本低、催化效率高、酶解工艺简化等显著优势,具有广阔的应用前景。作者首先介绍了不同植物来源木聚糖的结构组成及主链与侧链降解酶系,进而系统综述了近年来多功能木聚糖水解酶在结构、功能及作用机理方面的研究进展及在食品领域中的应用潜力,最后展望了多功能木聚糖水解酶的研究方向,为多功能木聚糖水解酶的精准设计及产业化应用奠定基础。Xylan is the most abundant heterogeneous polysaccharide in plant cell walls, which has great potential to synthesize high-value bio-based products. In the food industry, efficient enzymatic conversion of xylan can produce functional food polysaccharides such as xylo-oligosaccharides,xylitol and ferulic acid. Compared with the multi-enzyme synergistic catalysis, multifunctional enzymes combining multiple xylanolytic activities in one single peptide have significant advantages in reducing enzyme costs, improving catalytic efficiency and simplifying the enzymatic process, and therefore have broad application prospects. This paper firstly introduced the structural composition of xylan from different plant sources and the enzymatic systems for the degradation of main and side chains, then systematically reviewed the research progress of multifunctional xylanolytic enzyme with respect to structure, function and mechanism of action and their potential applications in the food industry, and finally prospected the research direction of multifunctional xylanolytic enzymes,laying a foundation for their precise design and industrial application.

关 键 词：木聚糖 多功能木聚糖水解酶 结构-功能关系 食品应用前景 

分 类 号：Q556.2[生物学—生物化学]