岩藻多糖对酒精暴露小鼠肝损伤的保护作用及机制  

Protective Effect and Mechanism of Fucoidan on Alcohol-Induced Liver Injury in Mice

作　　者：杨佳[1] 党凯 薛美兰[1] 梁惠[2] 张楠[1] 王青[3] 裴忠仟 秦益民 YANG Jia;DANG Kai;XUE Meilan;LIANG Hui;ZHANG Nan;WANG Qing;PEI Zhongqian;QIN Yimin(School of Basic Medicine,Qingdao University,Qingdao 266071,China;School of Public Health,Qingdao University,Qingdao 266071,China;Department of Ophthalmology,The Affiliated Hospital of Qingdao University,Qingdao 266021,China;State Key Laboratory of Bioactive Seaweed Substances,Qingdao Brightmoon Seaweed Group Co.,Ltd.,Qingdao 266555,China)

机构地区：[1]青岛大学基础医学院,山东青岛266071 [2]青岛大学公共卫生学院,山东青岛266071 [3]青岛大学附属医院眼科,山东青岛266021 [4]青岛明月海藻集团有限公司,海藻生物活性物质国家重点实验室,山东青岛266555

出　　处：《食品科学》2023年第3期137-145,共9页Food Science

基　　金：国家自然科学基金面上项目(81872605);山东省自然科学基金项目(ZR2020MH215);山东省重大科技创新项目(2019JZZY010818)。

摘　　要：目的:探讨岩藻多糖对酒精性肝病(alcoholic liver disease,ALD)的保护效果及潜在的分子机制。方法:用体积分数50%乙醇溶液建立ALD小鼠模型,再给予300 mg/kg mb岩藻多糖干预,比较对照组、酒精模型组与岩藻多糖干预组的肝组织形态学变化,测定血清转氨酶活力、血脂5项、炎性因子4项水平及辅助性T细胞(helper T cell,Th)1、Th2和Th17的比例,自噬蛋白表达水平和微管相关蛋白1轻链3β(microtubule-associated protein 1 light chain 3 beta,LC3B)荧光蛋白表达水平等指标,探究岩藻多糖对ALD的改善机制。结果:岩藻多糖可改善肝组织的不良病理变化,降低小鼠血清转氨酶(谷丙转氨酶、谷草转氨酶(aspartate aminotransferase,AST))和血脂(甘油三酯、总胆固醇、低密度脂蛋白胆固醇、总胆汁酸)水平(P<0.05),降低血清炎性因子(肿瘤坏死因子-α、白细胞介素(interleukin,IL)-1β和IL-6)和炎性细胞Th1、Th2和Th17的比例(P<0.05),下调自噬蛋白p62、哺乳动物雷帕霉素靶蛋白复合体1和核糖体蛋白70S6激酶等蛋白表达(P<0.05),促进转录因子EB的核转位(P<0.05),上调LC3B II蛋白和LC3B荧光蛋白的表达(P<0.05)。结论:岩藻多糖可改善ALD小鼠肝脏脂质毒性和炎症损伤,其作用机制可能与激活自噬有关。Objective:To investigate the protective effect and potential molecular mechanism of fucoidan on alcoholic liver injury.Methods:A mouse model of alcoholic liver disease(ALD)was established using 50%(V/V)alcohol,and then administered with 300 mg/kg mbof fucoidan.Liver histomorphological changes were compared in the control,ALD and fucoidan intervention groups,and the levels of serum aminotransferase,serum lipids,and inflammatory factors,the proportion of T helper(Th)cells including Th1,Th2 and Th17,and the expression levels of autophagy-related proteins and microtubule-associated protein 1 light chain 3 beta(LC3B)were detected to explore the mechanism by which fucoidan can improve alcoholic liver injury.Results:Fucoidan could improve the pathological changes of liver tissue,reduce the levels of serum alanine(aminotransferase(ALT)and aspartate aminotransferase(AST))and blood lipids(triglycerides(TG),total cholesterol(TC),low-density lipoprotein cholesterol(LDL-C)and total bile acid(TBA))(P<0.05),and decrease the levels of serum inflammatory factors(TNF-α,IL-1βand IL-6)and the proportion of Th1,Th2 and Th17(P<0.05).Meanwhile,it could down-regulate the expression of autophagy-related protein p62,mammalian target of rapamycin complex 1(mTORC1)and ribosomal protein 70S6 kinase(p70S6K)(P<0.05),promote the nuclear translocation of transcription factor EB(TFEB)(P<0.05),and up-regulate the expression of LC3B II protein and LC3B fluorescent protein(P<0.05).Conclusion:Fucoidan can alleviate lipotoxicity and inflammatory injury in the liver of ALD mice,and its mechanism may be related to the activation of autophagy.

关 键 词：自噬 酒精性肝病 岩藻多糖 血脂 炎症 

分 类 号：TS201.4[轻工技术与工程—食品科学]