不同光质处理对采后桃果皮色泽及花色苷代谢的影响  

Effects of Different Light Qualities on Color Development and Anthocyanin Metabolism of Peach Skin during Postharvest Storage

作　　者：池铭 孙丽娟 马立杰 赵婧[1] 周宏胜 凌军[1,2] 罗淑芬 李国锋 李鹏霞[1,2,3] 张映曈 CHI Ming;SUN Lijuan;MA Lijie;ZHAO Jing;ZHOU Hongsheng;LING Jun;LUO Shufen;LI Guofeng;LI Pengxia;ZHANG Yingtong(College of Food Science,Shenyang Agricultural University,Shenyang 110866,China;Key Laboratory of Cold Chain Logistics Technology for Agro-product,Ministry of Agriculture and Rural Affairs,Jiangsu Academy of Agricultural Sciences,Nanjing 210014,China;Jiangsu Key Laboratory for Horticultural Crop Genetic Improvement,Nanjing 210014,China)

机构地区：[1]沈阳农业大学食品学院,辽宁沈阳110866 [2]江苏省农业科学院,农业农村部农产品冷链物流技术重点实验室,江苏南京210014 [3]江苏省高效园艺作物遗传改良重点实验室,江苏南京210014

出　　处：《食品科学》2023年第3期209-217,共9页Food Science

基　　金：“十四五”国家重点研发计划项目(2022YFD2100601);江苏现代农业产业技术体系建设专项资金项目(JATS[2022]447);江苏省农业自主创新资金项目(CX(20)3046)。

摘　　要：为研究不同光质处理对桃果采后色泽的影响,探讨光照对桃果花色苷代谢的调控机制,本实验以‘中桃九号’为实验材料,在(22±1)℃条件下对桃果进行不同光照处理(红、绿、蓝、白光),光照时间12 h/d,以黑暗处理为对照。分析不同光照处理后桃果色泽、花色苷含量,及花色苷合成途径酶活力、结构基因和相关转录因子表达水平的变化。结果表明:红光、绿光对桃果皮着色没有明显影响,白光有微弱的促进作用,而蓝光能显著促进桃果皮色泽的提升,采后第6天时蓝光处理组桃果皮花色苷含量达到27.26mg/kg,分别是白光处理组和对照组的4.48倍和10.34倍。在蓝光作用下,花色苷合成途径酶活力以及结构基因和转录因子表达水平在大多数时间点显著高于对照组以及其他光照处理组(P<0.05)。其中,结构基因PAL、CHS、F3H、DFR、ANS、UFGT和转录因子MYB10.1的表达水平与桃果皮色泽参数以及花色苷含量呈显著正相关。综上,蓝光处理通过上调花色苷合成通路诱导桃果皮花色苷的合成和积累。研究结果将加深对光照调控植物花色苷代谢机制的理解,同时为研发桃采后色泽调控技术提供理论依据。The objective of this work was to study the effects of different light treatments on the color development of peach skin during postharvest storage and to explore the regulatory mechanism of light on anthocyanin metabolism.Peach fruits,cv.Zhongtao 9,were subjected to different lights(red,green,blue and white)for 12 h and darkness for another 12 h at(22±1)℃,while those kept in darkness for 24 h were considered as a control.The variations in fruit skin color,anthocyanin content and enzymatic activities involved in the anthocyanin metabolic pathway,as well as the expression levels of the structural genes of anthocyanin biosynthesis and related transcription factors after light treatment were measured.The results showed that the color development of peach skin was not affected by red and green light,but was weakly promoted by white light and significantly induced by blue light.The anthocyanin content in blue light-treated peach skin was 27.26 mg/kg,which was 4.48 and 10.34 times as high as that in the white light treatment and control groups on the sixth day after harvest,respectively.Under blue light treatment,the enzymatic activities of the anthocyanin biosynthesis pathway and the expression levels of structural genes and transcription factors were significantly higher than those in the control and other light treatment groups at most of the time points tested(P<0.05).The expression levels of the structural genes PAL,CHS,F3H,DFR,ANS and UFGT and the transcription factor MYB10.1 showed significantly positive correlation with the color parameters and anthocyanin content of peach skin.Collectively,this study indicated that blue light treatment promoted the synthesis and accumulation of anthocyanins in peach skin by up-regulating the anthocyanin synthesis pathway,which will deepen the understanding of the regulatory mechanism of anthocyanin metabolism by light in plants and provide a theoretical basis for the development of postharvest technologies to improve peach skin color.

关 键 词：桃 光照 色泽 花色苷 基因表达 

分 类 号：TS255.3[轻工技术与工程—农产品加工及贮藏工程]