不同抗氧化剂对皮肤抗氧化效果的对比研究  

Antioxidant effects of different antioxidants on skin

作　　者：吴昱萱 刘昌树 刘建国 李可欣 刘大松[1] 周鹏[1] WU Yuxuan;LIU Changshu;LIU Jianguo;LI Kexin;LIU Dasong;ZHOU Peng(State Key Laboratory of Food Science and Technology,Jiangnan University,Wuxi 214122,China;Standard Foods(China)Co.Ltd.,Suzhou 215000,China)

机构地区：[1]食品科学与技术国家重点实验室(江南大学),江苏无锡214122 [2]佳格食品(中国)有限公司,江苏苏州215000

出　　处：《食品与发酵工业》2023年第4期68-76,共9页Food and Fermentation Industries

基　　金：食品科学与技术国家一级学科计划项目(JUFSTR20180201)。

摘　　要：评价石榴提取物、雨生红球藻、针叶樱桃提取物和针叶樱桃提取物+维生素E对裸鼠皮肤抗氧化效果的影响。60只BALB/c Nude裸鼠随机分为6组:空白组、模型组和4组抗氧化物组,建立UVA+UVB联合紫外照射皮肤光老化模型。通过测定氧化还原稳态、胶原降解代谢、炎症和皮肤表型指标,综合分析不同抗氧化剂对皮肤抗氧化效果。结果显示,不同抗氧化剂对皮肤均有改善效果,其中雨生红球藻和针叶樱桃+维生素E效果较好,较模型组,雨生红球藻和针叶樱桃+维生素E显著减少活性氧、蛋白质羰基、白细胞介素-6含量,下调基质金属蛋白酶-1和12表达,降低皮肤a^(*)值,显著上调谷胱甘肽过氧化物酶表达。以上结果表明,雨生红球藻和针叶樱桃+维生素E具有改善皮肤抗氧化的效果,能延缓皮肤光老化。Overexposure to UV irradiation could increase reactive oxygen species that exceeds the defense capacity of endogenous antioxidant enzyme systems,and accumulate peroxidation products,resulting in an imbalance of the body's redox homeostasis.Further activation of related signaling pathways triggers collagen degradation and skin inflammation,ultimately leading to skin photoaging.It is manifested as a series of skin damage such as redness,dryness,lack of elasticity,and deepening of wrinkles.With the continuous improvement of aesthetic standards,the need to prevent and delay skin aging caused by UV irradiation is increasing.At present,improving antioxidant capacity is an important way to protect skin from UV irradiation-induced oxidative damage.Pomegranate extract is rich in polyphenols such as punicalagin and ellagic acid.Haematococcus pluvialis is one of the best raw materials for the production of astaxanthin.Acerola cherry can provide a variety of vitamins,especially vitamin C.As a fat-soluble antioxidant,vitamin E is often used with the water-soluble vitamin C.The above four substances are potential raw materials for antiphotoaging due to their antioxidant properties.The results of cell and biochemistry experiments in vitro often cannot be correlated to the antioxidant effect in vivo.The results from different experiments in vivo could not be compared directly to determine the difference in antioxidant effect and mechanism of different antioxidants on skin.Effects of pomegranate extract,H.pluvialis,acerola cherry extract and acerola cherry extract+vitamin E on skin antioxidant activity in mice was studied.Sixty male BALB/c nude mice were randomly divided into six groups:control,model,and four antioxidant groups,and an UVA+UVB irradiation-induced skin photoaging model was established.The model treatment lasted for two weeks,three times a week,and each time the UV irradiation dose was 5600 mJ/cm 2.The UVA and UVB irradiation intensity was 1.44 mW/cm 2 and 0.14 mW/cm 2,respectively.During the modeling period,the fou

关 键 词：石榴 雨生红球藻 针叶樱桃 维生素E 皮肤抗氧化 光老化 

分 类 号：TS201.4[轻工技术与工程—食品科学]