Global Profiling of 2-hydroxyisobutyrylome in Common Wheat  

作　　者：Ning Zhang Lingran Zhang Linjie Li Junyou Geng Lei Zhao Yan Ren Zhongdong Dong Feng Chen 

机构地区：[1]National Key Laboratory of Wheat and Maize Crop Science/Agronomy College,Henan Agricultural University,Zhengzhou 450046,China

出　　处：《Genomics, Proteomics & Bioinformatics》2022年第4期688-701,共14页基因组蛋白质组与生物信息学报（英文版）

基　　金：funded by the National Key R&D Program of China(Grant No.2019YFE0118300);the National Natural Science Foundation of China(Grant Nos.U1804234,31901542,and 31861143008);the Henan Natural Science Foundation,China(Grant No.202300410196)。

摘　　要：As a novel post-translational modification(PTM),lysine 2-hydroxyisobutyrylation(Khib)is considered to regulate gene transcriptional activities in eukaryotic cells;however,the functions of Khib-modified proteins in plants remain unknown.Here,we report that Khib is an evolutionarilyconserved PTM in wheat and its progenitors.A total of 3348 Khib sites on 1074 proteins are identified in common wheat(Triticum aestivum L.)by using affinity purification and mass spectroscopy of 2-hydroxyisobutyrylome.Bioinformatic data indicate that Khib-modified proteins participate in a wide variety of biological and metabolic pathways.Immunoprecipitation confirms that Khibmodified proteins are present endogenously.A comparison of Khib and other main PTMs shows that Khib-modified proteins are simultaneously modified by multiple PTMs.Using mutagenesis experiments and co-immunoprecipitation assays,we demonstrate that Khib on K206 of phosphoglycerate kinase(PGK)is a key regulatory modification for its enzymatic activity,and mutation on K206 affects the interactions of PGK with its substrates.Furthermore,Khib modification of low-molecular-weight proteins is a response to the deacetylase inhibitors nicotinamide and trichostatin.This study provides evidence to promote our current understanding of Khib in wheat plants,including the cooperation between Khib and its metabolic regulation.

关 键 词：Post-translational modification Lysine 2-hydroxyisobutyrylation Common wheat PROTEOMICS CO-IMMUNOPRECIPITATION 

分 类 号：S512.1[农业科学—作物学]