虾青素和/或有氧运动对D-半乳糖诱导大鼠心脏衰老的干预作用  

Effects of astaxanthin and/or aerobic exercise on D-galactose-induced myocardial aging in rats

作　　者：范妍君 胡海威[2] 曹建民[1] 周绮云[3,4] 胡戈 杨静晨 周海涛[3,4] 常平 FAN Yanjun;HU Haiwei;CAO Jianmin;ZHOU Qiyun;HU Ge;YANG Jingchen;ZHOU Haitao;CHANG Ping(Sport Science College of Beijing Sport University,Beijing 100084,China;Wangjing Hospital,China Academy of Chinese Medical Sciences,Beijing 100102,China;Beijing Union University,Beijing 100101,China;Key Laboratory of Bioactive Substances and Functional Foods,Beijing Union University,Beijing 100191,China;Physical Education College of Changzhou University,Changzhou,Jiangsu Changzhou 213164,China)

机构地区：[1]北京体育大学运动人体科学学院,北京100084 [2]中国中医科学院望京医院,北京100102 [3]北京联合大学,北京100101 [4]北京联合大学,生物活性物质与功能食品北京市重点实验室,北京100191 [5]常州大学体育学院,江苏常州213164

出　　处：《中国食品卫生杂志》2022年第6期1185-1192,共8页Chinese Journal of Food Hygiene

基　　金：北京联合大学科研项目(ZK10202210);北京联合大学“启明星”大学生科技创新创业项目(20212021)。

摘　　要：目的探究虾青素和/或有氧运动延缓D-半乳糖诱导大鼠心脏衰老的潜在作用机制。方法SPF级3月龄SD雄性大鼠40只随机分为安静对照组(C组)、急性衰老模型组(D组)、急性衰老+虾青素组(DA组)、急性衰老+有氧运动组(DE组)及急性衰老+虾青素+有氧运动组(DAE组),每组各8只。C组不进行任何干预,急性衰老各组大鼠腹腔注射100 mg/kg·d的D-半乳糖,同时分别以20 mg/kg·d虾青素和/或运动强度为60%最大摄氧量的有氧运动进行干预,实验周期为6周。末次训练12 h后取心脏,光学显微镜观察心脏组织形态并检测相关生化指标。结果心脏组织形态,C组正常;与C组比较,D组出现心肌细胞排列紊乱、心肌纤维断裂及炎性细胞浸润等现象;DA、DE、DAE心肌细胞排列整齐、心肌纤维断裂及炎性细胞浸润情况有所缓解,DAE组最为显著,心肌组织形态更接近于正常状态。与C组比较,D组心脏组织超氧化物歧化酶(SOD)、γ-谷氨酸半胱氨酸合酶(γ-GCS)活性及沉默信息调节因子1(SIRT1)、核因子E2相关因子2(Nrf2)、血红素氧合酶(HO-1)、B淋巴细胞瘤因子-2(Bcl-2)蛋白质表达水平、Bcl-2/Bcl-2相关X蛋白(Bax)比值显著降低(P<0.05或P<0.01);细胞凋亡水平、Bax蛋白质表达、丙二醛(MDA)含量显著升高(P<0.05或P<0.01)。与D组比较,DA组心肌组织SIRT1、Nrf2、HO-1蛋白质表达均显著升高(P<0.05),Bax蛋白质表达及MDA含量显著降低(P<0.05或P<0.01);DE组心肌组织SOD活性和SIRT1、HO-1蛋白质表达显著升高(P<0.05或P<0.01),Bax蛋白质表达及MDA含量显著降低(P<0.05或P<0.01);DAE组心肌组织SOD、γ-GCS活性及SIRT1、Nrf2、HO-1、Bcl-2蛋白质表达和Bcl-2/Bax比值显著升高(P<0.05或P<0.01),细胞凋亡水平、Bax蛋白质表达及MDA含量极显著降低(P<0.01)。与DA、DE组比较,DAE组心肌组织SIRT1、Bcl-2蛋白质表达及Bcl-2/Bax比值均显著升高(P<0.05或P<0.01),细胞凋亡水平显著降低(P<0.05);心肌组织细胞凋Objective To investigate the potential mechanism of astaxanthin and/or aerobic exercise in relieving Dgalactose-induced myocardial aging in rats.Methods Forty SD male rats aged 3 months were randomly divided into five groups,with 8 rats in each group:control group(group C),aging model group(group D),aging + astaxanthin group(group DA),aging + aerobic exercise group(group DE) and aging + astaxanthin + aerobic exercise group(group DAE).There was no intervention for group C.The other four groups were intraperitoneally injected with 100 mg/kg·d D-galactose.Group DA,DE and DAE were treated with 20 mg/kg·d astaxanthin and/or aerobic exercise at 60% VO2 max,respectively.The experiment lasted for 6 weeks.Twelve hours after the last training,the rats were sacrificed for heart harvesting.The morphology of the heart tissue was observed under light microscope and the related biochemical indicators were measured.Results The morphology of myocardium in group C was normal.Compared with group C,the myocardial cell in group D showed disorder,fiber rupture and inflammatory cell infiltration.Compared with group D,groups DA,DE and DAE showed improvement,including orderly arrangement of myocardial cells,relieved myocardial fiber rupture and reduction of inflammatory cells,especially in group DAE.and the morphology of myocardial tissue in group DAE was much closer to normal.Compared with group C,the activity of superoxide dismutase(SOD),γ-glutamate cysteine synthase(γ-GCs) and the expression of sirtuin 1(SIRT1),nuclear factor erythroid 2-related factor 2(Nrf2),heme oxygenase-1(HO-1) and Bcell lymphoma-2(Bcl-2) in myocardium and the ratio of Bcl-2/Bcl-2-associated X(Bax) of group D decreased significantly(P<0.05 or P<0.01);the apoptosis level,the expression of Bax and the malondialdehyde(MDA) content increased(P<0.05 or P<0.01).Compared with group D,the expressions of SIRT1,Nrf2 and HO-1 in group DA increased significantly(P<0.05),while the expression of Bax and the MDA content decreased significantly(P<0.05 or P<0.01).The activity

关 键 词：虾青素 有氧运动 衰老 氧化应激 SIRT1/Nrf2信号通路 大鼠 心脏 

分 类 号：R155[医药卫生—营养与食品卫生学]