消毒餐(饮)具中大肠菌群检测能力验证样品研制及其应用  

Development and application of coliform detection sample for proficiency testing on sterilized tableware

作　　者：石嵩 马师良 吕雨莎 张惠媛 李秋阳 姚瑶 SHI Song;MA Shiliang;LYU Yusha;ZHANG Huiyuan;LI Qiuyang;YAO Yao(Science and Technology Research Center of China Customs,Beijing 100026,China)

机构地区：[1]中国海关科学技术研究中心,北京100026

出　　处：《中国食品卫生杂志》2022年第6期1233-1237,共5页Chinese Journal of Food Hygiene

基　　金：国家重点研发计划(2019YFC1605100)。

摘　　要：目的 研制一种模拟消毒餐具采样涂抹过程的含菌棉拭子,应用于消毒餐饮具中大肠菌群检测能力验证。方法 阳性样品中含有目标菌和干扰菌,阴性样品仅含有干扰菌。随机抽取阴性、阳性样本各10瓶,按照GB 14934—2016附录B(发酵法)进行均匀性检验,将样品分别置于-20℃、4℃下贮藏120 d,在25℃、36℃和42℃下贮藏20~60 d监测其稳定性,检测结果应与指定值一致。发放3份样品,实验室最少收到1份阳性样品,其余随机发放。为防止数据串通,样本随机编号并缩短结果上报时间。结果 样品在均匀性、贮存稳定性和运输稳定性均能满足能力验证要求。通过考核,50家实验室评价满意,占91%;5家实验室评价不满意,占9%。结论 消毒餐饮具中大肠菌群检测能力验证样品可以满足能力验证要求,本次能力验证可真实反映参试单位的检测环境及检测水平。Objective To develop a bacteria-containing cotton swab which is applied to the proficiency testing for coliform detection on sterilized tableware.Methods The positive sample contained both target bacteria and interfering bacteria,and the negative sample only contained interference bacteria.10 bottles of negative and positive samples were selected randomly.The homogeneity tests were carried out in accordance with GB 14934—2016 Appendix B(Fermentation methed).The samples were also stored for 120 d at-20 ℃ and 4 ℃,and 20-60 d at 25 ℃,36 ℃ and 42 ℃respectively to monitor samples’ storage and transportation stability.Uniformity and stability testing results should be consistent with specific values.Three samples were delivered to each laboratory,and minimum of 1 positive sample was issued,the rest were randomly issued.In order to prevent data fabrication,sample numbers were randomly generated,and proficiency tests periods were shortened.Results The samples can meet the requirements of proficiency testing in terms of uniformity,storage stability and transportation stability.Fifty(91%) laboratories were qualified.Five(9%) laboratories were unqualified.Conclusion The proficiency test samples for the coliform detection on sterilized tableware can meet the requirements of proficiency testing.This proficiency test can truly reflect the testing environment and testing capability of the participating labortory.

关 键 词：大肠菌群 能力验证 消毒餐(饮)具 

分 类 号：R155[医药卫生—营养与食品卫生学]