酸浆宿萼中酸浆苦素的提取工艺及降糖活性  

Optimization of Extraction and in vitro Sugar-lowering Activity of Physalin from the Calyx of Physalis alkekengi L.

作　　者：李成华[1] 付艳艳[2] 薛长松[1] 陈建宇 朱芳娆 LI Cheng-hua;FU Yan-yan;XUE Chang-song;CHEN Jian-yu;ZHU Fang-rao(Laboratory for the Development and Utilization of Superior Crude Drug Resources in Changbai Mountain,Tonghua Normal University,Tonghua 134001,Jilin,China;Department of Nephrology,Affiliated Hospital of Changchun University of Chinese Medicine,Changchun 130021,Jilin,China)

机构地区：[1]通化师范学院长白山优势生药资源开发与利用实验室,吉林通化134001 [2]长春中医药大学附属医院肾病科,吉林长春130021

出　　处：《食品研究与开发》2023年第5期127-134,共8页Food Research and Development

基　　金：吉林省教育厅科学技术研究规划项目(JJKH20210532KJ);吉林省科技发展计划项目(20200404031YY);吉林省教育科学“十三五”规划项目(GH20298)。

摘　　要：该试验采用低共熔溶剂-超声波辅助提取法对酸浆宿萼中的酸浆苦素进行提取并进行降糖活性研究。该试验合成5种不同的低共熔溶剂,在单因素试验的基础上,采用四因素三水平的响应面法优化酸浆宿萼中的酸浆苦素的提取工艺条件,通过对α-葡萄糖苷酶、α-淀粉酶抑制能力测定评价酸浆苦素体外降糖活性。结果表明:氯化胆碱∶葡萄糖=2∶1(摩尔比)合成的低共熔溶剂酸浆苦素提取量最高,且明显高于75%乙醇组;最佳提取工艺为料液比1∶10(g/mL)、超声功率320 W、超声时间30 min、含水量15%,在该条件下,酸浆苦素提取量为(8.96±0.15)mg/g,酸浆苦素有降糖活性。Deep eutectic solvents(DESs)coupled with ultrasound-assisted extraction(UAE)was employed to extract physalin from the calyx of Physali alkekengi L. and the sugar-lowering activity of the extracted physalin was analyzed. Five different DESs were used for extraction and their performance was evaluated. On the basis of single factor tests,the response surface methodology was employed to optimize the extraction conditions of physalin from the calyx of P. alkekengi. The sugar-lowering activity of the extracted physalin was analyzed based on the inhibitory effects on α-glucosidase and α-amylase. The results showed that DESs(choline chloride ∶glucose = 2 ∶ 1) exhibited the highest extraction yield,which was significantly higher than that with 75%ethanol as the solvent. The conditions for physalin extraction were optimized as follows:solid-to-liquid ratio of1 ∶ 10(g/mL),ultrasonic power of 320 W,ultrasonic time of 30 min,and water content of 15%. Under these conditions,the yield of physalin extracted from the calyx of P. alkekengi was(8.96±0.15)mg/g. The physalin extracted from P. alkekengi had sugar-lowering activity.

关 键 词：酸浆宿萼 酸浆苦素 超声波 低共熔溶剂 提取 降糖活性 

分 类 号：TS201.2[轻工技术与工程—食品科学]