Investigation of paeonol-geniposide on acute alcoholic liver injury based on uniform design method  

作　　者：LIU Ke LIU Yu-long SUN Min LIU Ling-ling ZHANG Lu-ning YAN Gui-ming 

机构地区：[1]Anhui University of Chinese Medicine,Hefei 230012,China

出　　处：《Journal of Hainan Medical University》2022年第24期25-31,共7页海南医学院学报（英文版）

基　　金：Anhui Natural Science Foundation(No.2008085MH300)。

摘　　要：Objective:To explore the optimal ratio and compatibility effect of paeonol-geniposide combination on acute alcoholic liver injury by uniform design.Methods:Lieber-DeCarli alcoholic liquid feed was used to induce acute alcoholic liver injury in mice.Uniform design was used to select the best dosage combination of paeonol and geniposide,and the related indexes of liver injury and oxidative stress were detected by kit.Serum inflammatory factors were detected by ELISA,and the expressions of p38 MAPK,JNK and NF-κB P65 related proteins in liver were detected by Western-blot.Results:The regression equation suggested that paeonol:geniposide=220:20 was the best ratio of paeonol and geniposide to resist alcoholic liver injury.Compared with the model group,the liver injury indexes and oxidation products of the paeonol+geniposide group decreased significantly,the antioxidant activity of liver tissue increased significantly,and the expression levels of p-p38 MAPK,p-JNK and NF-κB P65 protein decreased significantly.Conclusion:The optimal dosage of paeonolgeniposide was effectively optimized by uniform design and pharmacodynamic analysis.The combination of the two drugs could reduce the alcoholic liver injury by reducing the oxidative stress injury and inflammatory response in the liver tissue of mice,and its effect might be related to the targeting of p38 MAPK/JNK/NF-κB channel.

关 键 词：PAEONOL GENIPOSIDE Alcoholic liver injury Uniform design Chinese medicine effective ingredient ratio 

分 类 号：R285[医药卫生—中药学]