ARMS-PCR技术在玫瑰果鉴别中的应用  

Application of ARMS-PCR Technology in Identification of Rosehip

作　　者：邵婕 杨正修 邹强 陆峥飞 李凡 SHAO Jie;YANG Zhengxiu;ZOU Qiang;LU Zhengfei;LI Fan(Herbalife Nat Source(Hunan)Natural Products Co.,Ltd.,Changsha 410100;Herbalife Nutrition,Global Quality,Torrance 90502)

机构地区：[1]康宝莱蕾硕(湖南)天然产物有限公司,长沙410100 [2]康宝莱全球测试中心HP1实验室,托伦斯90502

出　　处：《食品工业》2023年第1期161-165,共5页The Food Industry

摘　　要：玫瑰果(Rosa canina)是维生素C的重要来源,被广泛用于各种食品和膳食补充剂。刺玫果(Rosa davurica)原产于中国,与玫瑰果属于同一植物属,由于其果实表现出与玫瑰果相似的物理外观和植物化学特征,因此区分玫瑰果和刺玫果具有挑战性。开发一种用于玫瑰果原料鉴定的扩增阻滞突变系统-聚合酶链式反应方法 (ARMSPCR)。与物种特异性PCR相比,试验方法可同时靶向检测玫瑰果及其近亲物种刺玫果。经双盲试验验证该检测方法对玫瑰果的识别概率大于0.9 (95%CI),并证明检测刺玫果的最低检测限为10%。综上所述,该检测方法可用于快速鉴别玫瑰果和刺玫果。Rose hips(Rosa canina) are a great source of vitamin C and are used in a variety of foods and dietary supplements.Rosa davurica is native to China and belongs to the same genus as rose hips. Distinguishing rosehip from Rosa davurica is challenging as their fruits exhibit similar physical appearance and phytochemical characteristics. An amplification retardation mutation system-polymerase chain reaction method(ARMS-PCR) was developed for the identification of rosehip raw materials. Compared with species-specific PCR, this method could simultaneously targeted detect rose hips and its close relative, Rosa davurica. The double-blind experiment verified that the detection method had a recognition probability of rosehip greater than 0.9(95% CI), and proved that the detection sensitivity of rosehip was as low as 10%. The detection method can quickly identify rose hips and thorn rose hips.

关 键 词：玫瑰果 扩增阻滞突变系统-聚合酶链式反应 刺玫果 植物原料 

分 类 号：TS255.7[轻工技术与工程—农产品加工及贮藏工程]