IncR26319/miR-2834/EndophilinA axis regulates oogenesis of the silkworm,Bombyx mori  

作　　者：Yi Wang Yu Fu Hao Chena Chenyue Zhao Qunxia Huang Meiling Chang Wujie Qiu Yawen Shen Dandan Li 

机构地区：[1]Henan Key Laboratory of Insect Biology in Funiu Mountain,Henan International Joint Laboratory of Insect Biology,College of Life Science and Agricultural Engineering,Nanyang Normal University,Nanyang,Henan Province,China

出　　处：《Insect Science》2023年第1期65-80,共16页昆虫科学（英文版）

基　　金：supported byNational Natural Science Foundation of China(No.31970480);Natural Science Foundation of Henan province(No.212300410063);Young Elite Scientist Sponsorship Program by China Association for Science and Technol-0gy(YESS 20150026).

摘　　要：Oocyte maturation is critical for insect reproduction.Vitellogenesis,the timely production and uptake of vitellogenin(Vg),is crucial for female fecundity.Vg is synthesized in fat body and absorbed by the oocytes through endocytosis during insect oogenesis.In the silkworm,Bombyx mori,we discovered that a nucleus-enriched long-noncoding RNA(lncRNA)lncR26319 regulates Endophilin A(EndoA)-a member of the endophilin family of endocytic proteins-through competitive binding to miR-2834.The lncR26319-miR-2834-EndoA axis was required for Vg endocytosis in the silkworm;loss of EndoA or overexpression ofmiR-2834significantlyreduced eggnumbers invirginmoths.Inaddition,accumulation of miR-2834 resulted in pupal and adult deformation and reduced fecundity in females.The expression of Vg,30-kDa(30K)protein,and egg-specific protein(Esp)decreased after knockdown of EndoA or overexpression of miR-2834,while knockdown of miR-2834 had an opposite effect on the expression of Vg,30K protein gene,and Esp.These results suggest that the lncR26319-miR-2834-EndoA axis contributes to the endocytic activity in theVguptake and leads to the normal progression of oogenesis in the silkworm.Thus,miR-2834 and EndoA are crucial for female reproduction and could be potential targets for new pest management strategies in lepidopterans.

关 键 词：Bombyx mori ENDOPHILIN lncRNA miRNA OOGENESIS 

分 类 号：R73[医药卫生—肿瘤]