基于UPLC-MS/MS同时定量分析半夏不同炮制品中8种潜在毒性成分  

Simultaneous Quantitative Analysis of 8 Potential Toxic Components in Different Processed Products of Pinellia ternata Based on UPLC-MS/MS

作　　者：吕悦 张颖慧 孙宇婷 张良[5] 张琳[1,2,3,4] 祖元刚 Lü Yue;ZHANG Ying-hui;SUN Yu-ting(Key Laboratory of Forest Plant Ecology,Ministry of Education,School of Chemistry,Chemical Engineering and Eesource Utilization,Northeast Forestry University,Harbin,Heilongjiang 150040;Engineering Research Center of Forestry Biological Agents,Ministry of Education,School of Chemistry,Chemical Engineering and Resource Utilization,Northeast Forestry University,Harbin,Heilongjiang 150040;National Local Engineering Center for Ecological Utilization of Biological Resources,School of Chemistry,Chemical Engineering and Resource Utilization,Northeast Forestry University,Harbin,Heilongjiang 150040;Heilongjiang Provincial Key Laboratory of Ecological Utilization of Forestry-based Active Substances,Harbin,Heilongjiang 150040)

机构地区：[1]东北林业大学化学化工与资源利用学院森林植物生态学教育部重点实验室,黑龙江哈尔滨150040 [2]东北林业大学化学化工与资源利用学院林业生物制剂教育部工程研究中心,黑龙江哈尔滨150040 [3]东北林业大学化学化工与资源利用学院生物资源生态利用国家地方工程中心,黑龙江哈尔滨150040 [4]黑龙江省林源活性物质生态利用重点实验室,黑龙江哈尔滨150040 [5]黑龙江中医药大学附属第二医院南院,黑龙江哈尔滨150060

出　　处：《安徽农业科学》2023年第3期192-196,245,共6页Journal of Anhui Agricultural Sciences

基　　金：中央高校基本科研业务费专项基金项目(2572015CA06);林业公益性行业科研专项(201504701)。

摘　　要：[目的]建立超高效液相串联质谱(UPLC-MS/MS)法同时测定半夏生品及其炮制品中8种潜在毒性成分的含量,并分析其潜在毒性成分的差异。[方法]采用UPLC-MS/MS法测定半夏生品及其炮制品中8种潜在毒性成分的含量并进行方法学考察。流动相为甲醇-水溶液,梯度洗脱,流速为0.3 mL/min,质谱离子源为电喷雾离子源(ESI),检测模式为多反应监测(MRM),以苦参碱(正离子)和野黄芩苷(负离子)作为内标物。采用多元统计分析方法对8种潜在毒性成分的含量与炮制方法的相关性进行评价。[结果]8种潜在毒性成分在各自范围内线性关系良好(R^(2)≥0.999)。经多元统计分析,样品被聚集为4类,并通过变量权重重要性排序(VIP)发现胆碱、盐酸麻黄碱和原儿茶酸为4类样品间的主要差异成分。[结论]该方法快速、准确、灵敏度高、重现性好,可用于半夏生品及其炮制品中8种潜在毒性成分的含量测定,为后续分析提供参考。[Objective]To establish an ultra performance liquid chromatography tandem mass spectrometry(UPLC-MS/MS)method for the simultaneous determination of eight potentially toxic components in Pinellia ternata and its processed products,and to analyze the differences of their potentially toxic components.[Method]The UPLC-MS/MS method was used to determine 8 poential toxic components in different processed products of Pinellia ternata and to conduct a methodological investigation.The mobile phase was methanol-water solution,gradient elution,flow rate was 0.3 mL/min,mass spectrometry ion source was electrospray ionization source(ESI),detection mode was multiple reaction monitoring(MRM),matrine(positive ion)and scutellarin(negative ion)was used as the internal standard.The multivariate statistical analysis method was used to evaluate the correlation between the contents of the 8 toxic components and the processing methods.[Results]The 8 poential toxic components had a good linear relationship within their respective ranges(R^(2)≥0.999).After multivariate statistical analysis,the samples were clustered into 4 categories,and through variable weighted importance ranking(VIP),choline,ephedrine hydrochloride and protocatechuic acid were found to be the main differences among the 4 types of samples.[Conclusion]The method is rapid,accurate,sensitive and reproducible.It can be used for the determination of 8 potential toxic components in Pinellia ternata and its processed products,and provide reference for subsequent analysis.

关 键 词：半夏 UPLC-MS/MS 炮制品 毒性成分 含量测定 

分 类 号：R283[医药卫生—中药学]