响应面法优化板栗苞中抑制痢疾杆菌活性成分的提取工艺  

Extraction optimization by response surface methodology of active ingredients from chestnut burs for Shigella dysenteriae inhibition

作　　者：陈丽楠 彭飞 靳长迎[1] 余祚庆 杨越冬 CHEN Li'nan;PENG Fei;JIN Changying;YU Zuoqing;YANG Yuedong(Hebei Key Laboratory of Active Components and Functions in Natural Products,Qinhuangdao 066004,Hebei,China;Engineering Research Center of Chestnut Industry Technology,Ministry of Education,Qinhuangdao 066004,Hebei,China;Hebei Collaborative Innovation Center of Chestnut Industry,Qinhuangdao 066004,Hebei,China)

机构地区：[1]河北省天然产物活性成分与功能重点实验室,河北秦皇岛066004 [2]板栗产业技术教育部工程研究中心,河北秦皇岛066004 [3]河北省板栗产业协同创新中心,河北秦皇岛066004

出　　处：《精细化工》2023年第1期101-108,共8页Fine Chemicals

基　　金：河北省研究生创新资助项目(CXZZSS2022090)。

摘　　要：为提高板栗废弃物的资源利用率,以板栗苞为原料,探究其抑制痢疾杆菌活性成分的提取工艺。采用吸光光度法筛选板栗苞抑制痢疾杆菌活性成分的提取溶剂;以抑菌率为响应值,采用单因素实验结合响应面法优化板栗苞抑菌活性成分的提取工艺;采用二倍稀释法测定板栗苞各提取物的最低抑菌质量浓度(MIC),并采用吸光光度法研究其对细菌生长曲线的影响。结果表明,板栗苞的乙醇水提取物对痢疾杆菌的抑菌活性最好;板栗苞抑菌成分的最佳提取工艺为乙醇体积分数45%,料液比1∶20(g∶m L),提取温度50℃;在该条件下,板栗苞提取物抑菌率为61.27%±2.60%,MIC值为3.2 g/L;乙酸乙酯相提取物MIC值为0.4 g/L,抑菌率达85%以上。In order to improve the resource utilization rate of chestnut waste,the extraction process of active ingredient from chestnut burs against Shigella dysenteriae were explored.The extraction solvent was screened via spectrophotometry,and the extraction process was optimized by single factor experiment combined with response surface methodology with bacteriostatic rate as response value.The minimum inhibitory concentration(MIC)of extracts from chestnut burs were determined by double dilution method,and its effect on bacterial growth curve was analyzed by absorbance spectrophotometry.The results demonstrated that the extract from chestnut burs by ethanol and water mixture exhibited the best antibacterial activity against Shigella dysenteriae,with antibacterial rate of 61.27%±2.60% and MIC value of 3.2 g/L,under the optimal extraction conditions of ethanol and water mixture(ethanol volume fraction of 45%)as solvent,ratio of material to liquid 1∶20(g∶m L),extraction temperature 50℃.Meanwhile,the MIC value of ethyl acetate phase extract was 0.4 g/L,and the antibacterial rate was above 85%.

关 键 词：板栗苞 提取 抑菌活性 响应面优化 中药现代化技术 

分 类 号：TS201.3[轻工技术与工程—食品科学] R284[轻工技术与工程—食品科学与工程]