蛹虫草提取物对肾阳虚模型大鼠睾丸CYP19/CaM/CaMKⅡ信号的影响  

Effect of Cordyceps militaris extract on CYP19/CaM/CaMKⅡsignaling in testes of kidney yang deficiency model rats

作　　者：陈思源[1] 王思雨 祁明媛 李垄坡 徐仪昕 张婷[1] 姚紫研 赵洪庆[1] CHEN Siyuan;WANG Siyu;QI Mingyuan;LI Longpo;XU Yixin;ZHANG Ting;YAO Ziyan;ZHAO Hongqing(Hunan University of Chinese Medicine,Changsha 410208,China;Hunan Yandi Biological Engineering Co.,Ltd.,Zhuzhou 412000)

机构地区：[1]湖南中医药大学,长沙410208 [2]湖南炎帝生物工程有限公司,湖南株洲412000

出　　处：《中国比较医学杂志》2023年第1期1-8,共8页Chinese Journal of Comparative Medicine

基　　金：国家自然科学基金(82104836);湖南中医药大学产学研合作项目(2020430102003711)。

摘　　要：目的明确蛹虫草提取物补肾阳的功效及潜在作用机理。方法60只SD大鼠分为正常组、模型组、睾丸片组(0.216 g/kg)、蛹虫草提取物高、中、低剂量组(0.72、0.36、0.18 g/kg),每组10只。除正常组外,其余组均采用腺嘌呤(200 mg/kg)灌胃的方法建立肾阳虚大鼠模型,于造模第15天起开始给予受试药,连续28 d。采用ELISA试剂盒检测大鼠血清促性腺激素释放激素(GnRH)、黄体生成素(LH)、睾酮(T)、雌二醇(E2)水平,HE染色观察肾、睾丸、附睾、精囊腺的组织形态,免疫组化和Western blot法检测睾丸CYP19、CaM、CaMKⅡ表达,RT-qPCR检测睾丸CYP19 mRNA表达。结果与正常组相比,模型组大鼠血清GnRH、LH、T水平均显著降低(P<0.05),肾、睾丸、附睾、精囊腺组织均有明显损伤,睾丸CYP19表达降低(P<0.01)、CaM和CaMKⅡ表达显著增加(P<0.01),CYP19 mRNA表达显著降低(P<0.01);与模型组相比,蛹虫草提取物高剂量组GnRH、LH、T含量均升高(P<0.05),组织损伤缓解,睾丸CYP19表达增加(P<0.05),CaM和CaMKⅡ降低(P<0.05),CYP19 mRNA水平增加(P<0.05)。结论蛹虫草提取物通过调控CYP19/CaM/CaMKⅡ信号发挥补肾阳功效。Objective To clarify the efficacy and potential mechanism of Cordyceps militaris extract to nourish kidney yang.Methods SD rats were divided into normal,model,testicular tablet(0.216 g/kg),and Cordyceps militaris extract high,medium,and low dose groups(0.72,0.36 and 0.18 g/kg,respectively)with 10 per group.Except for the normal group,all other groups were administered adenine(200 mg/kg)by gavage to establish a kidney yang deficiency rat model.Test drug administration was started on day 15 of model establishment for 28 consecutive days.ELISA were used to measure rat serum gonadotropin releasing hormone(GnRH),luteinizing hormone(LH),testosterone(T)and estradiol(E2)levels.HE staining was used to observe the morphology of kidneys,testes,epididymis,and seminal vesicle glands.Immunohistochemistry and Western blot were used to detect CYP19,CaM and CaMKⅡexpression in testes.CYP19 mRNA expression was detected in testes by RT-qPCR.Results Compared with the normal group,serum GnRH,LH and T levels of the model group were significantly reduced(P<0.05),kidney,testis,epididymis and seminal vesicle gland tissues were all significantly damaged,and CYP19 expression in testes was reduced(P<0.01).CaMKⅡexpression was increased significantly(P<0.01).CYP19 mRNA expression was decreased significantly(P<0.01).Compared with the model group,GnRH,LH and T contents in the high-dose Cordyceps militaris extract group were increased(P<0.05),tissue damage was relieved,CYP19 expression in testis was increased(P<0.05),CaM and CaMKⅡwere decreased(P<0.05),and the CYP19 mRNA level was increased(P<0.05).Conclusions Cordyceps militaris extract exerts a kidney yang effect by regulating CYP19/CaM/CaMKⅡsignaling.

关 键 词：蛹虫草 肾阳虚 提取物 睾丸 CYP19 

分 类 号：R-33[医药卫生]