2017~2021年陕西省实验动物使用单位SPF级大、小鼠质量监测结果分析与评估  

Analysis and evaluation of quality monitoring results of SPF rats and mice in Shaanxi province from 2017 to 2021

作　　者：吴朋朋 张彩勤 赵勇 谭邓旭 师长宏 白冰 WU Pengpeng;ZHANG Caiqin;ZHAO Yong;TAN Dengxu;SHI Changhong;BAI Bing(Laboratory Animal Center,Air Force Medical University,Xi’an 710032,China)

机构地区：[1]空军军医大学实验动物中心,西安710032

出　　处：《中国比较医学杂志》2023年第1期70-77,共8页Chinese Journal of Comparative Medicine

基　　金：军队实验动物专项科研课题(SYDW[2018]08号);陕西省科技资源开放共享平台项目(2022PT-36)。

摘　　要：目的分析2017~2021年陕西省部分实验动物使用单位无特定病原体(specific pathogen free,SPF)级大、小鼠微生物和寄生虫质量检测结果,了解各单位大、小鼠病原微生物学质量状况,为实验动物质量管理与监测提供参考依据。方法按照现行国家标准GB 14922.2-2011、GB/T 14926-2001和GB/T 14926.21-2008,由陕西省实验动物质量监督检测中心对大、小鼠进行病原微生物和寄生虫抽样检测并分析质量状况。结果2017年~2021年累计抽检109家单位的小鼠2549只,45家单位的大鼠503只。检测结果显示,小鼠体外寄生虫(螨虫)检出率由5.26%降为0.15%,肠道鞭毛虫由10.53%降为0.45%,肠道蠕虫由0.40%升至2.39%,沙门菌由2.78%降至无检出,泰泽病原体抗体阳性率由1.38%降至无检出;大鼠体外寄生虫(螨虫)检出率由7.07%降至无检出;肠道鞭毛虫由11.50%降为0.83%,蠕虫由3.79%升至7.50%,金黄色葡萄球菌由1.77%降至无检出。小鼠病毒血清抗体阳性率,2017年检出小鼠肝炎病毒(4.71%)、仙台病毒(1.39%)和小鼠细小病毒(2.22%),2021年出小鼠肝炎病毒(0.30%)和仙台病毒(0.15%),小鼠肺炎病毒阳性率则由1.11%升至2.39%,小鼠呼肠孤病毒Ⅲ型由0.17%升至0.45%。大鼠仙台病毒阳性率由7.07%降为0.83%,大鼠细小病毒和大鼠冠状病毒仅2017年和2018年在个别单位有检出,大鼠肺炎病毒则由0.88%增长为10.00%。结论近5年的检测结果表明,陕西省实验动物使用单位的大、小鼠病原微生物质量逐年提升,但仍存在一定问题,应持续采取严格的生物安全防控措施,加强动物实验过程,确保各单位在实验周期内动物质量合格。Objective To determine the current pathogenic microbiology quality status of experimental rats and mice in Shaanxi province,we analyzed quality monitoring result from 2017 to 2021 to provide a reference for health screening of laboratory rats and mice.Methods Using the current national standards GB 14922.2-2011,GB/T 14926-2001,and GB/T 14926.21-2008,the Laboratory Animal Quality Supervision and Testing Center of Shaanxi carried out pathogenic microbial and parasite quality sampling tests on SPF rats and mice in all licensed companies and research institutes,and then analyzed the result.Results From 2017 to 2021,2549 SPF mice and 503 SPF rats were collected from 109 and 45 organizations,respectively.Mouse parasite detection showed a reduction of mites from 5.26%to 0.15%,intestinal flagellates from 10.53%to 0.45%,Salmonella and Tyzzer’s organism from 2.78%and 1.38%,respectively,to undetected.Intestinal helminths increased from 0.40%to 2.39%.In rats,there were declines in mites and Staphylococcus aureus from 7.07%and 1.77%,respectively,to undetected,intestinal flagellates from 11.50%to 0.83%,and enrichment of helminths from 3.79%to 7.50%.In terms of positive rates of mouse serum antibodies,in 2017,mouse hepatitis virus(4.71%),Sendai virus(1.39%),and minute virus of mice(2.22%)were positive.Additionally,in 2021,mouse hepatitis virus(0.30%)and Sendai virus(0.15%)were positive.These result indicated raised trends of both pneumonia virus of mice and reovirus type III.However,there was a reduction in Sendai virus from 7.07%to 0.83%and an increase of pneumonia virus from 0.88%to 10.00%.In rats,although parvovirus and coronavirus had predominant potentials,which were detected in the past 5 years,the number of infected organizations and the positive rate showed a downward trend annually.Conclusions The test result in the past 5 years showed that pathogenic microorganisms in rats and mice in Shaanxi province had improved yearly.However,many problems still existed.Therefore,strict biosafety prevention and control measures s

关 键 词：SPF级大、小鼠 质量监测 微生物学 寄生虫学 分析与评估 

分 类 号：R-33[医药卫生]