粘质沙雷氏菌Serratia Marcescens磷脂酶C在E.coli BL21(DE3)中表达及其在粗芝麻油脱胶中的应用  

Serratia Marcescens Phospholipase C Expressing in E.coli BL21 (DE3) and Application in Crude Sesame Oil Degumming

作　　者：王伟 王康林 杨培周[2] WANG Wei;WANG Kang-lin;YANG Pei-zhou(Hefei Kangnuo Biological Pharmaceutical Co.,Ltd,Hefei 231131,China;School of Food and Biological Engineering,Hefei University of Technology,Hefei 230009,China)

机构地区：[1]合肥康诺生物制药有限公司,安徽合肥231131 [2]合肥工业大学食品与生物工程学院,安徽合肥230009

出　　处：《药物生物技术》2022年第6期569-573,共5页Pharmaceutical Biotechnology

摘　　要：酶法脱胶因其安全、环保等优点而受到油脂加工业的关注。磷脂酶C(PLC)催化磷脂酰胆碱水解形成1,2-二酰基-sn-甘油和磷脂胆碱,PLC脱胶可以在不产生游离脂肪酸的情况下减少油渣产生并增加中性油产量。本研究将粘质沙雷氏菌PLC(Sm-PLC)基因整合进大肠杆菌BL21(DE3)中实现重组表达,该重组酶的相对分子质量大小约为80 000。Sm-PLC最适温度和pH值分别为50℃和7。在32℃下经1 mmol/L IPTG诱导4 h后,Sm-PLC的活性达到12.5 U/mL。Sm-PLC催化2.5 h后,粗芝麻油的磷含量从42.5 mg/kg降至4.9 mg/kg。磷脂酶Sm-PLC在植物油清洁脱胶中具有良好的应用潜力。Enzymatic degumming has been attracted the attention by oil processing industry due to its advantages of safety and environmental protection.The hydrolysis of a phosphatidylcholine is catalyzed by phospholipase C(PLC) to form 1,2-diacyl-sn-glycerol and phosphocholine.The production of oil residue is reduced and the output of neutral oil is increased by PLC degumming without the production of free fatty acids.In this study, Serratia marcescens PLC(Sm-PLC) was recombinantly expressed in engineered E.coli BL21(DE3) with the size of appropriately 80 000.The optimum temperature and pH value of Sm-PLC were 50 °C and 7,respectively.After the induction by 1 mmol/L IPTG for 4 h at 32 °C,the activity of Sm-PLC reached 12.5 U/mL.The phosphorus content of crude sesame oil decreased from 42.5 to 4.9 mg/kg after the catalysis of Sm-PLC for 2.5 h.Therefore, Sm-PLC had a potential in the clean processing of vegetable oils.

关 键 词：磷脂酶C 芝麻油 磷脂 酶法脱胶 粘质沙雷氏菌 油脂精炼 

分 类 号：Q556[生物学—生物化学] Q814.9