HPLC法同时测定甘薯叶中5种酚酸和异槲皮苷  

Simultaneous Determination of Five Phenolic Acids and Isoquercitrin in Sweet Potato(Ipomoea batatas(L.)Lam)Leaves by HPLC

作　　者：傅志丰 周鹤 石燕[2] 卢坚雯 FU Zhifeng;ZHOU He;SHI Yan;LU Jianwen(Food and Drug Inspection Sub Center of Jiujiang Inspection,Testing and Certification Center,Jiujiang 332000,China;Stare Key Laboratory of Food Science and Technology,Nanchang University,Nanchang 330047,China;Selenium Resources Development and Utilization Center of Yichun,Yichun 336000,China)

机构地区：[1]九江市检验检测认证中心食品药品检验检测分中心,江西九江332000 [2]南昌大学食品科学与技术国家重点实验室,江西南昌330047 [3]宜春市硒资源开发利用中心,江西宜春336000

出　　处：《食品工业科技》2023年第5期292-298,共7页Science and Technology of Food Industry

摘　　要：咖啡酸、绿原酸、3,4-二咖啡酰奎宁酸、3,5-二咖啡酰奎宁酸、4,5-二咖啡酰奎宁酸和异槲皮苷是甘薯叶中的主要活性成分,本研究建立高效液相色谱(high performance liquid chromatography,HPLC)同时测定这6种成分的方法。通过对检测波长、色谱柱、洗脱溶剂、洗脱流速等参数优化得到的最佳分析条件为:ZORBAX SB-C_(18)(4.6 mm×250 mm,5μm)为分析柱,以0.1%甲酸水溶液(A)和乙腈(B)为流动相,采用梯度洗脱:0~12 min,10%~25%B;12~18 min,25%B,流速0.8 mL/min,检测波长326 nm,柱温30℃。咖啡酸、绿原酸、3,4-二咖啡酰奎宁酸、3,5-二咖啡酰奎宁酸、4,5-二咖啡酰奎宁酸和异槲皮苷的线性范围分别为0.25~25.0、0.5~50.0、5.0~100.0、10.0~200.0、5.0~100.0和0.5~50.0μg/mL。该方法分析时间短(18 min),线性度(R^(2)≥0.998)、稳定性(RSD≤1.97%)、精密度(RSD≤1.56%)和加标回收率(95.14%~103.22%)好。对江西10个不同产地甘薯叶目标成分分析发现,宜春丰城的甘薯叶具有最高的总酚含量。In this study,a reversed-phase high performance liquid-chromatography coupled to ultraviolet detection(RPHPLC/UV)method for simultaneous determination of six major bioactive constituents(caffeic acid,chlorogenic acid,3,4-dicaffeoyl quinic acid,3,5-dicaffeoyl quinic acid,4,5-dicaffeoyl quinic acid and isoquercetin)in sweet potato leaves was developed and validated after optimization of various chromatographic conditions and other experimental parameters.The chromatographic separation was achieved on an Agilent ZORBAX SB-C_(18)reserved-phase column(4.6 mm×250 mm,5μm)using a gradient elution program with a mobile phase consisting of 0.1%formic acid aqueous solution(solvent A)and acetonitrile(solvent B)(0~12 min,10%~25%B;12~18 min,25%B),at a flow rate of 0.8 mL/min,an operating temperature of 30℃,and a wavelength of 326 nm.The calibration curves were linear in the range of 0.25~25.0,0.5~50.0,5.0~100.0,10.0~200.0,5.0~100.0,0.5~50.0μg/mL for caffeic acid,chlorogenic acid,3,4-dicaffeoyl quinic acid,3,5-dicaffeoyl quinic acid,4,5-dicaffeoyl quinic acid and isoquercetin,respectively.The present method was fast(18 min),and demonstrated acceptable values for linearity(R^(2)≥0.998),stability(RSD≤1.97%),recovery(95.14%~103.22%)and precision(RSD≤1.56%).Additionally,the new method was successfully applied to determine components in sweet potato leaves from ten different regions of Jiangxi Province,and the results indicated that the sample harvested in Fengcheng city of Yichun contained higher total phenol content than those collected in other regions.

关 键 词：HPLC 咖啡酰奎宁酸 异槲皮苷 同时测定 甘薯叶 

分 类 号：TS255.7[轻工技术与工程—农产品加工及贮藏工程]