HPLC同时测定天龙泉新款年份陶藏酒中17种活性成分  

Simultaneous Determination of Seventeen Active Ingredients in Novel Tianlongquan Pithos Stored Liquor by HPLC

作　　者：莫维 张龙 叶静萱 梁振荣 万瑞杰 张宇虹 杨金海 郝俊光 MO Wei;ZHANG Long;YE Jingxuan;LIANG Zhenrong;WAN Ruijie;ZHANG Yuhong;YANG Jinhai;HAO Junguang(College of Light Industry and Food Engineering,Guangxi University,Nanning 530000,China;Qinzhou Key Laboratory of Food Flavor Analysis and Control,College of Food Science and Engineering,Beibu Gulf University,Qinzhou 535011,China;Guangxi College and University Key Laboratory of High-value Utilization of Seafood and Prepared Food in Beibu Gulf,College of Food Engineering,Beibu Gulf University,Qinzhou 535011,China;Guangxi Tianlongquan Wine Co.,Ltd.,Luocheng 546400,China;Jin Pai Co.,Ltd.,Daye 435100,China;Institute of Science and Technology Information of Luocheng Mulao Autonomous County,Luocheng 546400,China)

机构地区：[1]广西大学轻工与食品工程学院,广西南宁530000 [2]北部湾大学食品工程学院钦州市食品风味分析与调控重点实验室,广西钦州535011 [3]广西高校北部湾海产品高值化利用与预制食品重点实验室北部湾大学食品工程学院,广西钦州535011 [4]广西天龙泉酒业有限公司,广西罗城546400 [5]劲牌有限公司,湖北大冶435100 [6]罗城仫佬族自治县科技情报研究所,广西罗城546400

出　　处：《食品工业科技》2023年第5期299-307,共9页Science and Technology of Food Industry

基　　金：广西壮族自治区科技计划项目(桂科AB21196002)。

摘　　要：目的:为满足企业对添加葛根、罗汉果、苦荞麦提取物的新款年份陶藏酒中活性成分含量的精准控制,建立高效液相色谱(HPLC)同时测定17种活性成分(3′-羟基葛根素、绿原酸、葛根素、表儿茶素、儿茶素、3′-甲氧基葛根素、葛根素芹菜糖苷、大豆苷、阿魏酸、芦丁、槲皮苷、异槲皮苷、山奈酚-3-O-芸香糖苷、罗汉果苷Ⅳ、罗汉果苷Ⅴ、槲皮素、山奈酚)的定量方法。方法:通过梯度优化使各组分有效分离,并对定量方法进行方法学考察。结果:采用Waters Atlantis^(■)T3色谱柱(4.6 mm×250 mm,5μm)色谱柱,以乙腈-0.1%磷酸水溶液为流动相,在此条件下各测定成分70 min内得到较好分离,不同活性成分相对应的定量波长为203、250、280、365 nm。定量方法重复性好RSD≤4.06%,精密度高RSD(relative standard deviation)≤3.86%,加标回收率准确可靠(平均加标回收率92.46%~106.25%,RSD≤4.56%)。结论:该方操作简单、高效、准确,适用于年份陶藏酒活性成分的品控。Objective:In order to satisfy the enterprise’s precise control the dosage of active ingredients in the new Tianlongquan pithos-stored liquor dosed with radix puerariae,siraitiae fructus and tartary buckwheat extracts.To establish a method for the simultaneous determination of 17 active ingredients(3’-hydroxypuerarin,chlorogenic acid,puerarin,epicatechin,catechins,3′-methoxypuerarin,puerarin apioside,daidzin,ferulic acid,rutin,quercetin,isoquercitrin,kaempferol-3-O-rutinoside,mogrosideⅣ,mogrosideⅤ,quercetin,kaempferol)by high performance liquid chromatography(HPLC).Methods:Through gradient optimization was carried out to separate each component effectively,and methodological investigations was performed for quantitation method.Results:The effective separation was achieved within 70 min on a Waters Atlantis^(■)T3 column(4.6 mm×250 mm,5μm)using a optimized gradient of mobile phase consisting of acetonitrile-0.1%phosphoric acid water,and with the quantitative wavelength was 203,250,280 and 365 nm correspondingly.This quantitative method was of with good repeatability(relative standard deviation RSD≤4.06%),high precision(RSD≤3.86%)and reliable recovery(average recoveries ranging from 92.46%to 106.25%,RSD≤4.56%).Conclusion:The method is simple,efficient and accurate,and suitable for the quality control of the active ingredients in pithos-stored liquor.

关 键 词：高效液相色谱法 活性成分 产品质量 天龙泉年份陶藏酒 

分 类 号：TS207.3[轻工技术与工程—食品科学]