ZNF3 regulates proliferation,migration and invasion through MMP1 and TWIST in colorectal cancer  

作　　者：Le Du Ning Liu Jianfeng Jin Meng Cao Yuantian Sun Xinzheng Gao Banzhan Ruan Shangfeng Yang Dongsheng Ge Yingzhuan Ye Yinxi Zhou Erfei Chen Jin Yang 

机构地区：[1]Hainan Provincial Key Laboratory of Carcinogenesis and Intervention,Department of Biology,Hainan Medical University,Haikou 571199,China [2]Department of Gastrointestinal Surgery,Hainan General Hospital,Hainan Affiliated Hospital of Hainan Medical University,Haikou 571199,China [3]Department of Biochemistry and Molecular Biology,Hainan Medical University,Haikou 571199,China [4]College of Biomedical Information and Engineering,Hainan Medical University,Haikou 571199,China [5]Key Laboratory of Resources Biology and Biotechnology in Western China,Ministry of Education,College of Life Sciences,Northwest University,Xi’an 710069,China [6]Institute of Preventive Genomic Medicine,Xi’an 710069,China

出　　处：《Acta Biochimica et Biophysica Sinica》2022年第12期1889-1896,共8页生物化学与生物物理学报（英文版）

基　　金：supported by the grants from the Hainan Provincial Natural Science Foundation of China(Nos.820RC632 and 2019RC203);the Scientific Research Project of Higher Education in Hainan Province(No.Hnky2020-34);the Open Project of Hainan Provincial Key Laboratory of Basic Medicine(Nos.JCKF2020002 and JCKF2020001);the Innovation and Entrepreneurship Training Program for college students of Hainan Medical University(No.202211810012);the Innovation Program of Young Scientific and Technological Talents of Hainan Association of Science and Technology(No.QCXM202017);the Natural Science Basic Research Plan in Shaanxi Province of China(No.2019ZDLSF02-05);Hainan Province Science and Technology Special Fund(Nos.ZDYF2021SHFZ245 and ZDYF2022SHFZ311).

摘　　要：Colorectal cancer(CRC)is a malignant tumor with a high incidence and mortality worldwide.Currently,the underlying molecular mechanisms of CRC are still unclear.Zinc finger protein 3(ZNF3)is a zinc-finger transcription factor that has been reported as a candidate for breast cancer prognosis,suggesting its involvement in the regulation of tumorigenesis.However,the association between ZNF3 and CRC remains unknown.To investigate the role of ZNF3 in CRC,we first analyze the correlation between ZNF3 expression and CRC,and the results demonstrate that ZNF3 is highly expressed in CRC tissue and cells,which is associated with the age of CRC patients.In vitro studies show that ZNF3 overexpression promotes CRC cell migration.Compared to control cells,knockdown of ZNF3 markedly suppresses CRC cell proliferation,migration and invasion and promotes G0/G1 phase cell cycle arrest.The expressions of the EMT-related markers TWIST and MMP1 are significantly decreased when ZNF3 is silenced.Additionally,overexpression of MMP1 and TWIST exacerbates CRC cell proliferation,accelerates the S phase cell cycle in ZNF3-knockdown SW480 cells,and increases cell migration and invasion through Transwell chambers.These data suggest that ZNF3 is involved in cellular proliferation,migration and invasion by regulating MMP1 and TWIST in CRC cells.

关 键 词：ZNF3 colorectal cancer PROLIFERATION MIGRATION INVASION 

分 类 号：R730[医药卫生—肿瘤]