Effects of plumbagin on migration and invasion of human hepatoma cell line via JAK2/STAT3 signaling pathway  

作　　者：CHENG Tao WEI Yan-fei LIU Huan LIU Hong DENG Shu-ye 

机构地区：[1]School of Basic Medical Sciences,Guangxi University of Traditional Chinese Medicine,Nanning 530200,China [2]Guangxi Key Laboratory of Molecular Biology of Preventive Medicine of Traditional Chinese Medicine,Nanning 530001,China

出　　处：《Journal of Hainan Medical University》2023年第1期33-41,共9页海南医学院学报（英文版）

基　　金：Program of Guangxi Natural Science Foundation(No.2019GXNSDA 245003);The second batch of"Qihuang Project"high-level talent team cultivation project of Guangxi University of Traditional Chinese Medicine(No.2021001);Doctoral Research Initiation Fund of Guangxi University of Traditional Chinese Medicine(2020BS027);2021 Guangxi Postgraduate Innovation Project(No.YCXJ2021007)。

摘　　要：Objective:To study the effect of plumbagin(PL)on the migration and invasion of human hepatocellular carcinoma(HCC)cells and its possible mechanism.Methods:The cell counting kit(CCK-8)was used to detect the effects of different concentrations of plumbagin on the proliferation of human hepatocellular carcinoma Huh-7 and LM3 cells.The effect of plumbagin on the migration ability of Huh-7 and LM3 cells was detected by scratch test and Transwell migration test,and the effect of on the invasion ability of Huh-7 and LM3 cells was detected by Transwell invasion test.Western Blot was used to detect the expression of E-cadherin,N-cadherin,matrix metalloproteinase-2 and related proteins in JAK2/STAT3 signaling pathway in Huh-7 and LM3 cells.Results:Plumbagin could inhibit the proliferation of Huh-7 and LM3 cells in a time-and concentration-dependent manner.Plumbagin inhibited the migration and invasion of Huh-7 and LM3 cells in a concentration dependent manner,and it can down-regulate the expression of N-cadherin and MMP-2 protein,up-regulate the expression of E-cadherin protein,and inhibit the activation of JAK2/STAT3 signaling pathway.Conclusion:Plumbagin can inhibit the migration and invasion of human hepatocellular carcinoma Huh-7 and LM3 cells,and the molecular mechanism of this process may be related to the inhibition of JAK2/STAT3 signaling pathway activation.

关 键 词：PLUMBAGIN Hepatic carcinoma JAK2/STAT3 signaling pathway Migration INVASION 

分 类 号：R73[医药卫生—肿瘤]