交叉引物扩增法检测食品中大肠杆菌不耐热肠毒素的探讨  

Detection of Heat Labile Enterotoxin of Escherichia coli in Food by Cross Primer Amplification

作　　者：李伟 任重杰 LI Wei;REN Zhongjie(Yuncheng County Inspection and Testing Center,Heze 274700,China)

机构地区：[1]郓城县检验检测中心,山东菏泽274700

出　　处：《食品安全导刊》2023年第6期169-171,共3页China Food Safety Magazine

摘　　要：CPA技术与常规PCR技术相比,既避免了对昂贵的仪器设备的依赖性,又能减少检测费用,且由于其特异性高、灵敏度高,可大幅度提高检测速度。本文基于交叉引物扩增法技术及应用,设计了使用该方法检测食品中大肠杆菌不耐热肠毒素的实验,以期能够为相关人员提供借鉴与帮助。Compared with the conventional PCR technology,CPA technology can not only avoid the dependence on expensive instruments and equipment,but also reduce the detection cost,and because of its high specificity and sensitivity,it can greatly improve the detection speed.Based on the technology and application of cross primer amplification,this paper designed an experiment using this method to detect heat labile enterotoxin of Escherichia coli in food,in order to provide reference and help for relevant personnel.

关 键 词：交叉引物扩增法 大肠杆菌 不耐热肠毒素 

分 类 号：TS207.5[轻工技术与工程—食品科学]