野生二粒小麦主要农艺特性融入普通小麦的全基因组关联分析  

Genome-wide association study for agronomic traits in common wheat lines derived from wild emmer wheat

作　　者：刘佳 龚方仪 刘亚西[1] 颜泽洪[1] 钟晓英 陈厚霖 黄林[1] 伍碧华[1] LIU Jia;GONG Fang-Yi;LIU Ya-Xi;YAN Ze-Hong;ZHONG Xiao-Ying;CHEN Hou-Lin;HUANG Lin;WU Bi-Hua(State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China/Triticeae Research Institute,Sichuan Agricultural University,Chengdu 611130,Sichuan,China)

机构地区：[1]西南作物基因资源发掘与利用国家重点实验室/四川农业大学小麦研究所,四川成都611130

出　　处：《作物学报》2023年第5期1184-1196,共13页Acta Agronomica Sinica

基　　金：四川省国际科技创新合作项目(2021YFH0110);四川省生物育种重大科技专项(2022ZDZX0014)资助。

摘　　要：野生二粒小麦(Triticum turgidum ssp.dicoccoides)是普通小麦的四倍体祖先种,具有广泛的基因型变异,是改良普通小麦的重要种质资源。本研究对不同年份和地点四个环境下的161份野生二粒小麦渗入系材料的株高、分蘖数、小穗数、抽穗期、开花期和千粒重进行表型鉴定,并利用覆盖全基因组的13,116个DArT标记对各农艺性状进行全基因组关联分析,以期发掘性状显著关联标记及相关候选基因。本研究共检测到147个与6个农艺性状相关的稳定标记。其中,一些关联标记与抽穗期和开花期同时相关,并且在2B染色体上聚集成簇。在野生二粒小麦渗入系群体中共推定了21个与农艺性状相关的候选基因,其中位于7A染色体与千粒重显著相关的候选基因与细胞周期蛋白有关。这些标记和候选基因可为克隆优异农艺性状相关基因提供重要信息,从而为野生二粒小麦在普通小麦背景中的遗传改良综合利用提供依据与指导。Wild emmer(Triticum dicoccoides ssp. dicoccoides) is the tetraploid progenitor of common wheat with wide genotypic variations, which is an important germplasm resource for common wheat improvement. In this study, the plant height, tiller number, spikelet number, heading date, anthesis date, and 1000-kernel weight of 161 introgression lines derived from wild emmer were phenotyped in four environments of different years and locations. We performed genome-wide association study(GWAS) of agronomic traits using 13,116 DArT markers to identify marker-trait associations(MTA) and candidate genes. A total of 147 stable markers associated with the tested 6 agronomic traits were identified. Among them,some MTAs were associated with both heading and anthesis date, and these MTAs were clustered on chromosome 2B. In addition, a total of 21 candidate genes related to the agronomic traits were deduced in introgression lines derived from wild emmer. Among them, the candidate gene associated with 1000-kernel weight on chromosome 7A maybe related to cyclin protein. These MTAs and candidate genes could provide the essential information for cloning genes related to excellent agronomic traits and further provide basis and guidance for comprehensive utilization of wild emmer for genetic improvement in common wheat background.

关 键 词：野生二粒小麦 普通小麦 农艺性状 远缘杂交 全基因组关联分析 

分 类 号：S512.1[农业科学—作物学]