基于GC-MS的脑出血代谢标志物研究  

Study on metabolic markers of intracerebral hemorrhage based on GC-MS

作　　者：冉丽 彭形[2] 吴玉洪 熊昕[1] RAN Li;PENG Xing;WU Yuhong;XIONG Xin(Department of Neurology,Chongqing Hospital of Traditional Chinese Medicine,Chongqing 400011,China;Department of Neurosurgery,Chongqing Hospital of Traditional Chinese Medicine,Chongqing 400011,China;Chongqing Hospital of Traditional Chinese Medicine,Chongqing 400011,China)

机构地区：[1]重庆市中医院神经内科,重庆400011 [2]重庆市中医院神经外科,重庆400011 [3]重庆市中医院,重庆400011

出　　处：《现代医药卫生》2023年第5期725-729,共5页Journal of Modern Medicine & Health

基　　金：重庆市科学技术局科研机构绩效激励引导专项项目(jxyn2019-2-1);国家中医药管理局中医药循证能力建设项目(2019XZZX-NB014)。

摘　　要：目的探索脑出血与血浆代谢标志物之间的关系及可能的机制。方法收集2020年3-12月就诊于重庆市中医院神经内科、神经外科的住院患者,发病24 h内就诊并完成头颅CT平扫确诊为脑出血,同期健康体检者为对照组。采用气相色谱-质谱联用技术(GC-MS)分析两组间血浆代谢物含量的变化,应用正交偏最小二乘法(OPLS-DA)、ROC曲线筛选出两组中的差异性代谢物。结果该研究共纳入脑出血组24例,对照组30例,两组的人口学特征无显著差异,共筛选出24个差异性标志物,进行ROC曲线分析,最终筛选出AUC>0.85的代谢物有4个:3-羟基丙酸、2-羟基丁酸、7-脱氢胆固醇、柠檬酸。结论3-羟基丙酸、2-羟基丁酸、7-脱氢胆固醇、柠檬酸等血浆代谢物可能与脑出血的发病相关,其中2-羟基丁酸、7-脱氢胆固醇可能与脑卒中后抑郁的发生有关。Objective To explore the relationship and potential mechanism between intracerebral hemorrhage(ICH)and plasma metabolomic biomarker and its possible mechanism.Methods The inpatients were enrolled in the study who underwent computed tomography(CT)scans within 24 hours of onset and were diagnosed as ICH from Department of Neurology and Neurosurgery,Chongqing Hospital of Traditional Chinese Medicine,from March to December 2020.The healthy persons in the same period were taken as the control group.The changes of the plasma metabolite content between the two groups were analyzed by gas chromatography-mass spectrometry(GC-MS).The differential metabolites in two groups were screened by partial least squares discrimination analysis(OPLS-DA)and ROC curve.Results A total of 24 ICH patients and 30 cases of the control group were enrolled in the study,and their clinical characteristics were no significant difference.A total of 24 important metabolites were identified and analyzed by ROC curve.Finally,four differential metabolites were screened for predicting ICH by applying the area under curve(AUC)greater than 0.85,including 3-hydroxypropionic acid,2-hydroxybutanoic acid,7-dehydrocholesterol and citric acid.Conclusion 3-hydroxypropionic acid,2-hydroxybutanoic acid,7-dehydrocholesterol and citric acid may be related to the pathogenesis of ICH,among which 2-hydroxybutanoic acid and 7-dehydrocholesterol may be related to the occurrence of the post-stroke depression.

关 键 词：脑出血 中风 代谢组学 生物标志物 气相色谱-质谱联用技术 

分 类 号：R741[医药卫生—神经病学与精神病学] R743[医药卫生—临床医学]