红树来源真菌Daldinia eschscholtzii HJ004发酵产物中三个新的次级代谢产物  

Three New Secondary Metabolites from the Mangrove-Derived Fungus Daldinia eschscholtzii HJ004

作　　者：王斌 曾尾女 李杲钰 肖媚 韦方芳 罗由萍[1,2] 钮智刚[1,2] 黄国雷 郑彩娟 Wang Bin;Zeng Wei-nü;Li Gao-yu;Xiao Mei;Wei Fang-fang;Luo You-ping;Niu Zhi-gang;HuangGuo-lei;Zheng Cai-juan(Key Laboratory of Tropical Medicinal Resource Chemistry of Ministry of Education,College of Chemistry and Chemical Engineering,Hainan Normal University,Haikou,571158,People’s Republic of China;Key Laboratory of Tropical Medicinal Plant Chemistry of Hainan Province,College of Chemistry and Chemical Engineering,Hainan Normal University,Haikou,571158,People’s Republic of China)

机构地区：[1]海南师范大学化学与化工学院,热带药用资源化学教育部重点实验室,海口571158 [2]海南师范大学化学与化工学院,海南省热带药用植物化学重点实验室,海口571158

出　　处：《有机化学》2023年第1期332-337,共6页Chinese Journal of Organic Chemistry

基　　金：海南省重点研发计划(No.ZDYF2021SHFZ270);国家自然科学基金(No.32160108,41866005);海南省自然科学基金(No.220RC593);海南省科技攻关计划(No.ZDKJ202008);海南省院士创新平台海南省专项(No.YSPTZX202030)资助项目。

摘　　要：从红树来源真菌Daldinia eschscholtzii HJ004中分离得到3个新化合物以及8个已知化合物.通过高分辨质谱、一维和二维核磁共振波谱以及X射线单晶衍射数据确定了8-乙基-7-羟基-5-羧基-2,3-二甲基色酮(1)的结构.通过电子圆二色谱(ECD)量子化学计算以及圆二色谱(CD)数据与文献进行比较,确定了felinone C (2)和螺旋霉素G (3)的绝对构型.利用总抗氧化能力检测试剂盒(ABTS快速法)测试了所得11个化合物的自由基清除能力,其中4个化合物表现出显著的抗氧化活性,IC_(50)值的范围为5.57~195.03μmol/L,强于阳性对照水溶性维生素E(trolox)的抗氧化活性(IC_(50)=292.12μmol/L),具有开发为抗氧剂的潜力.Three new compounds, together with eight known compounds, were isolated from the mangrove-derived fungus Daldinia eschscholtzii HJ004. The structure of 8-ethyl-7-hydroxy-5-carboxyl-2,3-dimethylchromone(1) was extensively elucidated and characterized by HRESIMS, 1D and 2D NMR, and single-crystal X-ray crystallography. The absolute configurations of felinone C(2) and helicascolide G(3) were determined by using electronic circular dichroism(ECD) method or comparing circular dichroism(CD) data with the literature. The radical scavenging capabilities of the 11 compounds obtained were tested by total antioxidant capacity assay kit with a rapid ABTS method. Among them 4 compounds exhibited stronger antioxidant activity with IC_(50) values range from 5.57 μmol/L to 195.03 μmol/L, than the positive control trolox(IC_(50)=292.12 μmol/L). These results indicate that chromones have the potential values of developing antioxidants.

关 键 词：Daldinia eschscholtzii 色原酮 内酯类化合物 抗氧化活性 

分 类 号：TQ920.1[轻工技术与工程—发酵工程]