黄鱼鱼鳔肽分离及其诱导前列腺癌DU-145细胞凋亡的机制  

Isolation of Peptide from Yellow Croaker Swim Bladder and Its Mechanism of Inducing Apoptosis of Prostate Cancer DU-145 Cells

作　　者：彭东 赖玉健 田柬昕 钟碧銮 安苗青 黎攀 杜冰[1,3] Peng Dong;Lai Yujian;Tian Jianxin;Zhong Biluan;An Miaoqing;Li Pan;Du Bing(College of Food Science,South China Agricultural University,Guangzhou 510642;Guangdong Shenzhiyuan Health Technology Co.Ltd.,Guangzhou 510000;SCAU and Guanzhan Fish Maw Nutrition and Security Research Center,Guangzhou 510642)

机构地区：[1]华南农业大学食品学院,广州510642 [2]广东参之源健康科技有限公司,广州510000 [3]华南农业大学与官栈鱼胶营养安全研究中心,广州510642

出　　处：《中国食品学报》2023年第2期72-82,共11页Journal of Chinese Institute Of Food Science and Technology

基　　金：广东省自然科学基金面上项目(2020A151501268);财政部和农业农村部:国家现代农业产业技术体系资助(CARS-21)。

摘　　要：为探讨黄鱼鱼鳔肽诱导前列腺癌DU-145细胞凋亡的作用机制,从黄鱼鱼鳔酶解产物中分离纯化得到鱼鳔肽,鉴定其氨基酸序列。采用CCK-8法检测鱼鳔肽作用后DU-145细胞抑制率的变化;AO/EB法检测DU-145细胞凋亡情况;流式细胞术检测DU-145细胞凋亡率和周期;免疫印记法检测DU-145细胞凋亡蛋白表达。结果表明,分离得到的鱼鳔肽YCSB-1c中包含Ser-Pro-Ser-Pro和Gly-Pro-Ala-Arg两条寡肽。与空白组相比,YCSB-1c组中凋亡细胞明显增多,且呈质量浓度依赖性;流式细胞仪检测得出YCSB-1c组中存活细胞明显减少,晚期凋亡细胞明显增多。YCSB-1c将DU-145细胞的细胞周期阻滞在G0/G1期,上调Bax、Caspase-3及Caspase-9蛋白表达,诱导DU-145细胞凋亡。YCSB-1c能有效诱导DU-145细胞凋亡,其作用机制与细胞周期停滞和线粒体介导的凋亡途径有关。The study aimed to investigate the mechanism of the peptide from yellow croaker swim bladder inducing apoptosis in prostate cancer DU-145 cells.Isolation and purification of the peptides from the enzymatic hydrolysate of yellow croaker swim bladder and identification of its amino acid sequences.The CCK-8 method was used to measure the inhibition rate of swim bladder peptide after treatment with YCSB-1c.AO/EB method was used to determine apoptosis in DU-145 cells.Flow cytometry was used to determine apoptosis rate and cell cycle in DU-145 cells,and western blot was used to determine the expression levels of apoptosis-related proteins.The results showed that the YCSB-1c contains two oligopeptides,Ser-Pro-Ser-Pro and Gly-Pro-Ala-Arg.Compared with the control group,the apoptotic cells in the YCSB-1c groups were significantly increased in a dose-dependent manner.Flow cytometry results showed that live cells in YCSB-1c groups were significantly decreased,while the late apoptotic cells were significantly increased.YCSB-1c blocked the cell cycle of DU-145 cells in G0/G1 phase,up-regulated the protein expression of Bax,Caspase-3 and Caspase-9,and induced DU-145 cells apoptosis.YCSB-1c could effectively induce apoptosis in DU-145 cells,and the underlying mechanism is related to cell cycle block and mitochondria-mediated apoptotic pathway.

关 键 词：黄鱼鱼鳔 抗前列腺癌肽 DU-145细胞 凋亡机制 

分 类 号：TS254.9[轻工技术与工程—水产品加工及贮藏工程]