绵羊EST-SSR分子标记的鉴定及特异性分析  

Identification and Specificity Analysis of EST-SSR Molecular Markers in Sheep

作　　者：马小梅[1] 宋亚倩 罗瑞明[1] Ma Xiaomei;Song Yaqian;Luo Ruiming(School of Food and Wine,Ningxia University,Yinchuan 750021)

机构地区：[1]宁夏大学食品与葡萄酒学院,银川750021

出　　处：《中国食品学报》2023年第2期330-340,共11页Journal of Chinese Institute Of Food Science and Technology

基　　金：宁夏回族自治区创新平台专项(2019DPC05011)。

摘　　要：通过SSR Finder软件及MISA技术从NCBI系统中的绵羊表达序列标签(EST)数据库中筛查SSR位点,利用Primer 3.0设计绵羊EST-SSR荧光引物,对宁夏滩羊肉、新疆细毛羊肉和藏绵羊肉进行区分。以3种绵羊的肝脏DNA为模板,对设计的EST-SSR引物进行PCR扩增并使用毛细管电泳检测SSR分型。结果显示,通过EST-SSR标记设计的荧光引物p2105.1:248-559、p2025.1:896-1059和p2104.1:704-1015在宁夏滩羊肉、新疆细毛羊肉和藏绵羊肉中具有良好特异性,可实现3个绵羊品种的区分。通过EST-SSR标记技术可准确判断3个纯种绵羊肉的品种,这对绵羊遗传资源的开发利用、品种鉴别以及丰富分子标记类型具有重要的意义。In this paper,SSR sites were screened from the sheep expression sequence tag in NCBI system(EST)database by SSR Finder software and MISA technology,and sheep EST-SSR fluorescent primers were designed using Primer 3.0 to differentiate the products of Ningxia Tan lamb,Xinjiang fine-wool sheep meat and Tibetan lamb.Experiments were PCR amplified using liver DNA from three sheep as templates for designed EST-SSR primers and detected SSR typing using capillary electrophoresis.The results showed that the fluorescent primers p2105.1:248-559,p2025.1:896-1059 and p2104.1:704-1015 designed by EST-SSR labeling showed good specificity in Ningxia Tan mutton,Xinjiang fine lamb and Tibetan mutton,indicating that the distinction of three sheep varieties was achieved.This study accurately judged the varieties of three pure-bred mutton through EST-SSR marker technology science,which was of important significance for the development and utilization of sheep genetic resources,variety identification and rich molecular marker types.

关 键 词：绵羊 EST-SSR 引物设计 特异性分析 

分 类 号：S826[农业科学—畜牧学]