杨木纤维细胞与薄壁细胞酶解性能差异性研究  

The Difference of Enzymatic Hydrolysis Performance BetweenPoplar Fiber Cells and Parenchyma Cells

作　　者：李名路 王岚[1,2] 陈洪章 LI Minglu;WANG Lan;CHEN Hongzhang(State Key Laboratory of Biochemical Engineering,Beijing Key Laboratory of Biomass Refining Engineering,Institute of Process Engineering,Chinese Academy of Sciences,Beijing 100190,China;School of Chemical Engineering,University of Chinese Academy of Sciences,Beijing 100190,China)

机构地区：[1]中国科学院过程工程研究所,生化工程国家重点实验室,生物质炼制工程北京市重点实验室,北京100190 [2]中国科学院大学化学工程学院,北京100190

出　　处：《生物质化学工程》2023年第2期21-28,共8页Biomass Chemical Engineering

基　　金：国家重点研发计划资助项目(2019YFB1503800);中国科学院洁净能源先导科技专项(XDA21060300)。

摘　　要：以杨木为原料,经冰醋酸-过氧化氢处理得到杨木纤维细胞和薄壁细胞,加入纤维素酶进行酶水解,考察了纤维细胞与薄壁细胞酶解性能的差异性,并对酶水解过程的影响因素、水分分布状态规律和力学性能进行分析。研究结果表明:薄壁细胞具有更高酶促反应可及性,酶解72 h可发酵糖达到208.6 g/L,较纤维细胞提高8.5%。在相同木糖得率(59%)下,薄壁细胞酶用量比纤维细胞降低了50%;在高固体系(固形物的量大于15 g/mL)下,薄壁细胞的酶解率提高了15.4%~33.3%。水分分布状态表明,薄壁细胞在酶解过程中水束缚作用更弱,具有更好的液化性能。根据力学性能分析发现酶水解过程的前24 h是决定纤维细胞与薄壁细胞酶解性能差异的关键阶段。此外,薄壁细胞在酶解过程中力学性能较弱,有助于节约能耗和缩短转化周期。In this study,fiber cells and parenchyma cells of poplar were prepared from poplar by glacial acetic acid-hydrogen peroxide treatment.On this basis,the differences of enzymatic hydrolysis between fiber cells and parenchyma cells were investigated,and the influencing factors,water distribution and mechanical properties of the enzymatic hydrolysis process were analyzed.The results showed that parenchyma cells had higher accessibility of enzymatic reaction and the fermentable sugar content of parenchyma cells reached 208.6 g/L after 72 h of enzymatic hydrolysis,which was 8.5%higher than that of fibrous cells.With the same enzymatic hydrolysis efficiency of xylose(59%),the enzyme loading of parenchyma cells decreased by 50%compared to fibrous cells.The enzymatic hydrolysis rate of parenchyma cells increased by 15.4%-33.3%with the high solid system(the solid loading was more than 15 g/mL).The state of water distribution showed that parenchyma cells had weaker water binding effect during enzymatic hydrolysis process and had better liquefaction performance.The mechanical properties showed that the key stage determining the difference of enzymatic hydrolysis performance between fiber cells and parenchyma cells was the first 24 h.In addition,parenchyma cells had weak mechanical properties during enzymatic hydrolysis,which helped to save energy and shorten cycle time.

关 键 词：纤维细胞 薄壁细胞 酶水解 水分分布 力学性能 

分 类 号：TQ35[化学工程]