荷叶和罗布麻总黄酮的富集纯化工艺研究  

Study on enrichment and purification technology of total flavonoids from lotus leaves and Apocynum venetum L.

作　　者：韦琴 刘清怡 范康俊 梅辉[2] WEI Qin;LIU Qing-yi;FAN Kang-jun;MEI Hui(College of Environmental and Biological Engineering,Wuhan Technology and Business University,Wuhan 430065,Hubei,China;College of Life Sciences and Technology,Wuhan University of Bioengineering,Wuhan 430415,Hubei,China)

机构地区：[1]武汉工商学院环境与生物工程学院,湖北武汉430065 [2]武汉生物工程学院生命科学与技术学院,湖北武汉430415

出　　处：《粮食与油脂》2023年第3期81-84,88,共5页Cereals & Oils

基　　金：湖北省资源环境与智能化工程优势特色学科(群)建设专项资金资助项目;校级学术团队特色生物资源开发与利用(No.XSTD2015003)。

摘　　要：以静态吸附率和解吸率为指标,筛选出适合荷叶、罗布麻总黄酮纯化的最佳大孔树脂,再用其进行动态吸附考察;采用单因素试验和正交试验优化荷叶、罗布麻总黄酮的纯化工艺条件。结果表明:荷叶、罗布麻总黄酮纯化的最佳树脂均是AB-8型大孔树脂,荷叶总黄酮最佳纯化工艺条件为上样流速3.0 BV/h、上样质量浓度1.606 mg/mL、乙醇体积分数80%、上样量1.4 BV,在此条件下荷叶总黄酮含量为65.75%;罗布麻总黄酮最佳纯化工艺条件为上样流速1.5 BV/h、上样质量浓度2.394 mg/mL、乙醇体积分数80%、上样量1.5 BV,在此条件下罗布麻总黄酮含量为46.75%。Based on the static adsorption rate and desorption rate, the best macroporous resin suitable for the purification of total flavonoids from lotus leaf and Apocynum venetum L. was selected, and then its dynamic adsorption was investigated. The purification conditions of total flavonoids from lotus leaf and Apocynum venetum L. were optimized by single factor test and orthogonal test. The results showed that the best resin for the purification of total flavonoids from lotus leaf and Apocynum venetum L. were AB-8 macroporous resin. The best purification conditions of total flavonoids from lotus leaf were as follows: the flow rate of sample was 3.0 BV/h, the mass concentration of sample was 1.606 mg/mL, the volume fraction of ethanol was 80 %, and the volume of sample was 1.4 BV. Under these conditions, the total flavonoids content of lotus leaf was 65.75 %. The optimal purification conditions of total flavonoids from Apocynum venetum L. were as follows: the flow rate of sample was 1.5 BV/h, the mass concentration of sample was 2.394 mg/mL, the volume fraction of ethanol was 80 %, and the volume of sample was 1.5 BV. Under these conditions, the total flavonoids content of Apocynum venetum L. was 46.75 %.

关 键 词：大孔树脂 荷叶 罗布麻 总黄酮 纯化 

分 类 号：TS201.1[轻工技术与工程—食品科学]