耐硒菌株Cytobacillus firmus N4的筛选及其对α-葡萄糖苷酶抑制活性的影响  

Screening of Selenium Tolerant Strain Cytobacillus firmus N4 and Preliminary Effect of Its Inhibitory Activity againstα-Glucosidase

作　　者：王一丹 余雍和 杨锐 段玉桦 周欣 王璋倩 WANG Yidan;YU Yonghe;YANG Rui;DUAN Yuhua;ZHOU Xin;WANG Zhangqian(College of Life Science and Technology,Wuhan Polytechnic University,Wuhan 430023,China;School of Modern Industry for Selenium Science and Engineering,Wuhan Polytechnic University,Wuhan 430040,China)

机构地区：[1]武汉轻工大学生命与科学技术学院,湖北武汉430023 [2]武汉轻工大学硒科学与工程现代产业学院,湖北武汉430040

出　　处：《食品与生物技术学报》2023年第2期45-50,共6页Journal of Food Science and Biotechnology

基　　金：国家自然科学基金项目(31900011);湖北省科技创新重大专项项目(2019ABA113);武汉轻工大学科研项目(2019y05);武汉轻工大学大学生科研项目(xsky2020028);湖北省“楚天学者计划”人才项目。

摘　　要：微生物制备纳米硒具有低毒、温和等优点,具有广阔的应用前景。从武汉施加硒肥的土壤中分离纯化得到一株能将亚硒酸钠还原成纳米硒的菌株,对其进行菌种鉴定,测定其耐硒能力和生长动力学曲线,利用扫描电镜对制备的纳米硒颗粒进行表征,并将转化得到的纳米硒及菌株次生代谢产物对α-葡萄糖苷酶的抑制活性进行初步探究。结果表明,在分离纯化的16株菌株中,坚强芽孢杆菌N4(Cytobacillus firmus N4)对亚硒酸钠的最大耐受性为36 mg/mL,产生的纳米硒粒径为150~220 nm。纳米硒质量浓度为0.625 mg/mL时对α-葡萄糖苷酶的抑制率为(25.42±0.69)%;次生代谢产物质量浓度为2.500 mg/mL时,抑制率达到(73.83±4.63)%。Cytobacillus firmus N4具有高耐硒性质,可为纳米硒的生物转化提供基础,且其次生代谢产物有一定的α-葡萄糖苷酶抑制活性,值得进一步深入研究。The preparation of selenium nanoparticles from microorganism had low toxicity,mild synthesis and other advantages,showing broad application prospects.A strain which could reduce sodium selenite to selenium nanoparticles was isolated and purified from the soil with supplemental selenium fertilizer in Wuhan.The strain was identified,and its selenium tolerance and growth kinetics curve were determined.The selenium nanoparticles were characterized by scanning electron microscope,and the inhibitory activity of selenium nanoparticles and the secondary metabolites onα-glucosidase were preliminarily explored.The results showed that among the 16 strains isolated and purified,the maximum tolerance concentration of Cytobacillus firmus N4 to sodium selenite was 36 mg/mL with the selenium nanoparticle size of 150~220 nm.When the concentration of selenium nanoparticles was 0.625 mg/mL,the inhibition rate ofα-glucosidase was(25.42±0.69)%,and the inhibition rate reached to(73.83±4.63)%when the concentration of secondary metabolites was 2.500 mg/mL.Cytobacillus firmus N4 has high selenite-tolerance,providing the basis for the biotransformation of selenium nanoparticles,and its secondary metabolites show certainα-glucosidase inhibitory activity,which is worthy of further study.

关 键 词：耐硒菌株 纳米硒 坚强芽孢杆菌 α-葡萄糖苷酶抑制活性 

分 类 号：TQ92[轻工技术与工程—发酵工程]