低共熔溶剂预处理对笋壳木质素结构及物化性质的影响  

Effect of deep eutectic solvent pretreatment on the structure and physicochemical properties of lignin from bamboo shoot shell

作　　者：胡强 王延云[2] 龚卫华[3] 王燕 唐梦劼[2] HU Qiang;WANG Yan-yun;GONG Wei-hua;WANG Yan;TANG Meng-jie(Bamboo Diseases and Pests Control and Resources Development Key Laboratory of Sichuan Province,Leshan Normal University;College of Life Science,Leshan Normal University,Leshan 614000,China;National and Local United Engineering Laboratory of Integrative Utilization of Eucommia ulmoides,Jishou University,Jishou 416000,China)

机构地区：[1]乐山师范学院竹类病虫防控与资源开发四川省重点实验室 [2]乐山师范学院生命科学学院,乐山614000 [3]吉首大学杜仲综合利用技术国家地方联合工程实验室,吉首416000

出　　处：《天然产物研究与开发》2023年第3期365-371,共7页Natural Product Research and Development

基　　金：乐山师范学院“科研培育计划”(2022SSDJS017,2022SSDJS002);四川省高等教育人才培养质量和教学改革项目(JG2021-1252,JG2021-1255)。

摘　　要：以慈竹(Neosinocalamus affinis)笋壳为原料,采用氯化胆碱和乳酸低共熔溶剂预处理提取氯化胆碱-乳酸(choline chloride-lactic acid,CC-LA)木质素,并对木质素结构、热稳定性及抗氧化活性进行表征和分析。结果表明:CC-LA木质素的得率在37.67%~62.32%之间,远高于磨木木质素的得率(13.4%)。CC-LA木质素相对分子质量低,多分散性指数小于2,结构均一性好。在氯化胆碱与乳酸的物质的量之比为1∶3,处理温度为130℃条件下得到CC-LA 3130木质素,该木质素较其他CC-LA木质素和磨木木质素醇羟基含量最低,酚羟基含量最高,热稳定性最好,清除DPPH自由基活性最强,自由基清除指数为3.77,高于商业抗氧化剂2,6-二叔丁基对甲酚(2.11),本研究说明了氯化胆碱/乳酸在最佳条件下(1∶3,130℃)提取的木质素具有应用于抗氧化剂领域的潜力。Choline chloride-lactic acid(CC-LA)lignin was extracted from the shells of bamboo shoots of Neosinocalamus affinis with choline chloride and lactic acid deep eutectic solvent.The structure,thermal stability and antioxidant activity of lignin were characterized and analyzed.The results showed that the yield of CC-LA lignin ranged from 37.67%to 62.32%,which was much higher than that of milled wood lignin(13.4%).CC-LA lignin has low relative molecular mass,polydispersity index less than 2,and good structural homogeneity.CC-LA 3130 lignin was prepared at 130℃with the molar ratio of choline chloride to lactic acid of 1∶3.Compared with other CC-LA lignin and milled wood lignin,CC-LA 3130 lignin had the lowest alcohol hydroxyl content,the highest phenolic hydroxyl content,the best thermal stability,and the strongest scavenging activity of DPPH,and the free radical scavenging index is 3.77,which is higher than the commercial antioxidant butylated hydroxytoluene(2.11).This study demonstrates that lignin extracted from choline chloride/lactic acid under optimal conditions(1∶3,130℃)has the potential to be used in the field of antioxidants.

关 键 词：低共熔溶剂 笋壳 木质素 结构 热稳定性 抗氧化性 

分 类 号：O636.2[理学—高分子化学] R914[理学—化学]