表面增强拉曼光谱法快速测定小麦表面的脱氧雪腐镰刀菌烯醇  

Rapid detection of deoxynivalenol in wheat surface based surface enhanced Raman spectroscopy

作　　者：康牧旭 武晓莉 张宗绵 吕学冲 张建新 郭华 张红芬 张雪峰 KANG Mu-Xu;WU Xiao-Li;ZHANG Zong-Mian;LV Xue-Chong;ZHANG Jian-Xin;GUO Hua;ZHANG Hong-Fen;ZHANG Xue-Feng(Xingtai Institute of Food and Drug Inspection,Xingtai 054000,China;Xingdong New Area of Xingtai Ecological Environment Bureau,Xingtai 054000,China;Institute of Environmental Health and Ecological Security,School of the Environment and Safety Engineering,Jiangsu University,Zhenjiang 212013,China;Baoding Institute of Product Quality Inspection,Baoding 071000,China)

机构地区：[1]邢台市食品药品检验所,邢台054000 [2]邢台市生态环境局邢东新区分局,邢台054000 [3]江苏大学环境与安全工程学院,环境健康与生态安全研究院,镇江212013 [4]保定市产品质量监督检验所,保定071000

出　　处：《食品安全质量检测学报》2023年第4期17-23,共7页Journal of Food Safety and Quality

基　　金：河北省市场监督管理局科研计划重点项目(2021ZD41);江苏大学高级人才科研启动基金项目(16JDG033)。

摘　　要：目的基于表面增强拉曼光谱法(surface enhanced Raman spectroscopy,SERS)建立小麦表面脱氧雪腐镰刀菌烯醇(deoxynivalenol,DON)的快速检测方法。方法以银纳米颗粒(silver nanoparticles,AgNPs)为基础,利用其表面修饰的特殊官能团与DON之间的疏水作用,捕获小麦表面附着的DON,然后再以AgNPs作为表面增强拉曼基底材料,在铝箔表面进行自组装并进行SERS检测。结果采用1-硫代硅烷对AgNPs进行修饰,可以更好地捕获DON分子,选择合适浓度的AgNPs在铝箔表面进行自组装就可以实现DON的快速SERS检测。实验结果显示,在DON浓度为3.3~333.0mg/L的范围内具有良好的线性关系。将经过修饰的AgNPs分散到小麦表面,可以捕获附着在其表面的DON,回收率实验显示,在小麦表面DON的添加浓度为12、25和50μg/g时,均可以实现80%以上的回收率。结论本方法操作简单、快速,结合便携式拉曼光谱仪,有望用于小麦表面DON的快速现场检测。Objective To establish a rapid detection method of deoxynivalenol(DON) on wheat surface based on surface enhanced Raman spectroscopy(SERS). Methods Based on silver nanoparticles(AgNPs), the special functional groups modified on the surface of AgNPs were used to capture the DON attached to the wheat surface by using the hydrophobic interaction between DON and the special functional groups modified on the surface of AgNPs. Then AgNPs were used as the SERS material to conduct self-assembly on the aluminum foil surface and SERS detection. Results The modification of AgNPs with 1-thiosilane could better capture DON molecules, and the self-assembly of AgNPs with appropriate concentration on the aluminum foil surface could achieve fast SERS detection of DON. The experimental results showed that there was a good linear relationship in the range of DON concentration from 3.3 to 333.0 mg/L. Dispersing the modified AgNPs onto the wheat surface could capture the DON attached to the wheat surface. The recovery experiment showed that the recovery rate of more than 80% could be achieved after the addition of DON on wheat surface with the concentrations of 12, 25 and 50 μg/g.Conclusion This method is simple and fast. Combined with the portable Raman spectrometer, it can be used for rapid field detection of DON on wheat surface.

关 键 词：表面增强拉曼光谱法 纳米银 小麦 脱氧雪腐镰刀菌烯醇 

分 类 号：O657.37[理学—分析化学] TS211.7[理学—化学]