冠突散囊菌SP-5固态发酵普洱大叶毛茶产果胶酶的研究  

Solid-state fermentation to Pu-erh big leaf tea with Eurotium cristatum SP-5 to produce pectinase

作　　者：岳彦超 杨云娟[1,3] 郝大海 黄遵锡[1,3] 唐湘华 YUE Yanchao;YANG Yunjuan;HAO Dahai;HUANG Zunxi;TANG Xianghua(College of Life Sciences,Yunnan Normal University,Kunming 650500,China;Yunnan Key Laboratory of Potato Biology,Kunming 650500,China;Key Lab of Yunnan Province for Biomass Energy and Environmental Biotechnology,Kunming 650500,China)

机构地区：[1]云南师范大学生命科学学院,云南昆明650500 [2]云南省马铃薯重点实验室,云南昆明650500 [3]云南省生物质能与环境生物技术重点实验室,云南昆明650500

出　　处：《食品与发酵工业》2023年第6期162-169,共8页Food and Fermentation Industries

基　　金：云南省教育厅自然科学基金资助项目(2020J0091)。

摘　　要：该文利用BIOLOG工作站分析了冠突散囊菌对75种碳源的利用,通过显色反应筛选出可利用的碳源,以碳源结构设计多聚体底物,用于冠突散囊菌诱导培养定向产酶,筛选新酶产品。结果表明,采用果胶、木聚糖、纤维素作为碳源接种培养冠突散囊菌SP-5,其菌落形态生长良好。根据冠突散囊菌SP-5利用果胶诱导产酶特性,我们对普洱的大叶毛茶固态发酵获得果胶酶并进行了酶学性质分析。结果表明,冠突散囊菌SP-5在培养基含水量为20%~40%的比例中长势较好,其中,在含水量20%的茶叶培养基中产生的果胶酶活力最高,能达到12.52 U/g;果胶酶最适反应温度为50.0℃,最适pH为3.50;在80℃条件下的半衰期为30.28 min,在pH 2.0条件下半衰期为15.39 min;动力学研究发现SP-5果胶酶米氏常数K_(m)为1.21 g/L,酶促最大反应速率v_(max)为6.15μmol/(U·min)。In the BIOLOG system,75 kinds of carbon sources were used for Eurotium cristatum SP-5 to screen the new enzyme species.The available carbon sources were screened out through chromogenic reactions.The polymeric substrates were designed based on the carbon source structure,which could be used in the induction culture of E.cristatum for directing enzyme production.E.cristatum SP-5 was used for solid-state fermentation to produce pectinase.The results showed that pectin,xylan,and cellulose were used as carbon sources to culture SP-5,and its colony morphology grew well.Based on the pectinase induced by SP-5 with pectin,solid-state fermentation was used to produce the enzyme by SP-5 with Pu-erh big leaf tea of Yunnan,and its enzymatic dynamics were analyzed.The experiments showed that E.cristatum SP-5 grew best in the pectin medium and had the highest pectinase activity in tea medium with 20%water content,reaching 12.5 U/g.The optimum reaction temperature and pH of pectinase from SP-5 were 50℃and 3.5,respectively.The half-life of pectinase′s heat and acid resistance was 30.28 min at 80℃and 15.3 min at pH 2.0.The K_(m)of SP-5 pectinase was 1.21 mg/mL,and the maximum reaction rate v_(max)was 6.15μmol/(U·min).Metal ion magnesium,copper,iron,calcium,potassium,and silver ions have inhibitory effects on pectinase.

关 键 词：BIOLOG工作站 冠突散囊菌 果胶酶 动力学 半衰期 

分 类 号：TS272.5[农业科学—茶叶生产加工]