三唑类杀菌剂的免疫检测技术研究进展  

Research progress of immunoassays for detection of triazole fungicides

作　　者：金雅琪 彭怡霖 赵其阳[1,2] 张耀海 焦必宁[1,2] 崔永亮 JIN Yaqi;PENG Yilin;ZHAO Qiyang;ZHANG Yaohai;JIAO Bining;CUI Yongliang(Key Laboratory of Quality and Safety Control of Citrus Fruits,Ministry of Agriculture and Rural Affairs,Southwest University,Chongqing 400712,China;National Citrus Engineering Research Center,Citrus Research Institute,Southwest University,Chongqing 400712,China)

机构地区：[1]西南大学,农业农村部柑橘类果品质量安全控制重点实验室,重庆400712 [2]西南大学,柑桔研究所,国家柑桔工程技术研究中心,重庆400712

出　　处：《食品与发酵工业》2023年第6期315-323,I0002-I0004,共12页Food and Fermentation Industries

基　　金：国家农产品质量安全风险评估项目(GJFP20210204);国家现代农业(柑桔)产业技术体系建设专项(CARS-26)。

摘　　要：三唑类杀菌剂是一类有机含氮杂环类化合物,兼具保护、治疗作用而被广泛应用于水果、蔬菜、烟草等农产品生产中。近年来,随着食品物流行业发展,三唑类杀菌剂在采后保鲜、贮藏和运输领域的应用也越来越普遍。但其半衰期长、不易降解,容易造成食品中残留超标问题,影响人类健康和进出口贸易。因此,实现三唑类杀菌剂的高效快速检测,对食品安全和产业健康发展具有重要意义。利用抗原-抗体结合的免疫检测技术具有高效、灵敏等优点,被广泛应用于食品中农药残留的快速检测。该文以三唑类杀菌剂的免疫检测方法为出发点,总结了当前国内外三唑类杀菌剂的半抗原的设计合成、完全抗原和抗体的制备进展,并分类介绍了已开发的三唑类杀菌剂的免疫检测方法(主要包括酶联免疫分析法、免疫层析试纸条、荧光免疫分析法和免疫亲和色谱法),为今后开发更为快速简便的三唑类杀菌剂的残留检测方法提供参考。Triazole fungicides,as a class of organic nitrogen-containing heterocyclic compounds,are widely used in the production of agricultural products such as fruits,vegetables,and tobacco due to protective and curative functions.For the past few years,with the development of the food logistics industry,the application of triazole fungicides in post-harvest preservation,storage,and transportation has increased significantly.However,triazole fungicides have a long half-life and low degradation rate,which could lead to excessive pesticide residues in food,affecting human health,as well as the import and export trade.Therefore,the development of efficient and rapid detection methods for triazole fungicides is of great significance to food safety and industrial development.Based on the specific interaction between antibodies and antigens,immunoassays have the advantages of high efficiency and sensitivity,and have been widely used in the rapid detection of pesticide residues in food.This paper reviewed the progress in the design and synthesis of haptens and preparation of antigens and antibodies for triazole fungicides,and introduced immunoassay methods for triazole fungicides(including enzyme-linked immunosorbent assay,immunochromatography assay,fluorescence immunoassay,and immunoaffinity chromatography),which would be beneficial to the development of rapider and simpler detection methods for triazole fungicides in the future.

关 键 词：三唑类杀菌剂 农药残留 免疫检测 半抗原 抗体 

分 类 号：TS207.5[轻工技术与工程—食品科学]