单孢杂交选育金针菇新品种‘上研1820’  

Breeding of a new Flammulina filiformis cultivar‘Shangyan 1820’by single spore hybridization

作　　者：王瑞娟[1] 陆欢[1] 徐珍[1] 宋春艳[1] 谭琦[1] 刘建雨 尚晓冬[1] WANG Ruijuan;LU Huan;XU Zhen;SONG Chunyan;TAN Qi;LIU Jianyu;SHANG Xiaodong(Key Laboratory of Applied Fungal Resources and Utilization(South),Ministry of Agriculture of China,National Engineering Research Center of Edible Fungi,Shanghai Key Laboratory of Agricultural Genetics and Breeding,Institute of Edible Fungi,Shanghai Academy of Agricultural Sciences,Shanghai 201403,China)

机构地区：[1]上海市农业科学院食用菌研究所、农业农村部应用真菌资源与利用重点开放实验室、囯家食用菌工程技术研究中心、上海市农业遗传育种重点实验室,上海201403

出　　处：《菌物学报》2023年第2期597-608,共12页Mycosystema

基　　金：上海市科技兴农项目[沪农科创字(2020)第1-3号]。

摘　　要：本研究以收集的105份金针菇种质资源经遗传性分析后,选出‘上研1号’与‘0747’菌株作为亲本,采用单孢杂交技术共得到168个杂交菌株。经实验室初筛和工厂化小中试复筛,最终获得产量高、出芽整齐和商品性状优良的金针菇新品种‘上研1820’。该品种为浅黄色,菌盖呈球形,菌柄基部颜色加深不显著,子实体干品氨基酸总含量为1.82×10^(4)mg/kg。工厂化栽培条件下,菌丝最适培养温度为20℃,子实体生长发育温度为5–15℃,瓶栽菌丝培养周期为22d,子实体生育时间为25d,平均单瓶产量为314.3g/瓶。研究结果表明,利用分子标记技术和传统选育手段,可有效提高育种效率,对筛选出具有遗传性状稳定、商品性状优良和适用于工厂化栽培等特性的目标菌株具有重要的理论指导意义。A total of 105 germplasm resources of Flammulina filiformis was genetically analyzed by using molecular markers.‘Shangyan 1’and‘0747’strains were selected as parents,and 168hybrids were obtained by single spore hybridization.After primary screening in the laboratory and re-screening in the small and medium-sized factory test,a new cultivar of F.filiformis,‘Shangyan 1820’,with high yield,neat buds and excellent commercial properties was finally obtained.The cultivar is light yellow,with spherical cap,insignificant color deepening at the base of stipe,and the total amino acid content of the dried fruiting body is 1.82×10^(4)mg/kg.Under factory cultivation conditions,the optimum culture temperature of mycelia is 20℃,the growth temperature of fruiting body was 5–15°C,the mycelium culture period of bottle culture was 22 d,the fruiting body growth time was 25 d,and the average yield per bottle is 314.3 g/bottle.The research results show that the use of molecular marker technology and traditional breeding methods can effectively improve the breeding efficiency and select target strains with stable genetic properties and excellent commercial traits suitable for industrial cultivation.

关 键 词：金针菇 品种选育 单孢杂交 

分 类 号：S64[农业科学—蔬菜学]