响应面法优化解脂亚罗酵母产菜油甾醇培养基组成  

Optimization of medium composition for campesterol production in Yarrowia lipolytica by response surface methodology

作　　者：谭思远 陈月星 雷秋燕 佘佳怡 孟永宏[3] TAN Siyuan;CHEN Yuexing;LEI Qiuyan;SHE Jiayi;MENG Yonghong(School of Biomedicine and Food Engineering,Shangluo University,Shangluo 726000,Shaanxi,China;Shaanxi Qinling Industrial Technology Research Institute of Special Biological Resources,Shangluo 726000,Shaanxi,China;College of Food Engineering and Nutritional Science,Shaanxi Normal University,Xi'an 710119,China)

机构地区：[1]商洛学院生物医药与食品工程学院,陕西商洛726000 [2]陕西秦岭特色生物资源产业技术研究院,陕西商洛726000 [3]陕西师范大学食品工程与营养科学学院,西安710119

出　　处：《中国油脂》2023年第2期85-90,共6页China Oils and Fats

基　　金：中央引导地方科技发展专项(2019ZY-FP-02);商洛学院校级项目(19SKY014)。

摘　　要：为提高解脂亚罗酵母PMEDD菌株菜油甾醇的产量,对此菌株发酵培养基组成进行了优化。采用单因素实验探究碳源种类、氮源种类、碳源质量浓度、氮源质量浓度、KH2PO4质量浓度对菜油甾醇产量的影响。在此基础上,采用响应面实验对发酵培养基组成进行了优化。结果表明:培养基组成为葡萄糖3.17 g/100 mL,蛋白胨1.44 g/100 mL,KH2PO40.65 g/100 mL,Uracil 0.1 g/100 mL,YNB 0.42 g/100 mL,MgSO4·7H2O 0.15 g/100 mL时,菜油甾醇的产量达到最大,为16.49μg/mL,优化后菜油甾醇的产量为优化前的2.10倍。采用优化后的培养基,可有效提高解脂亚罗酵母PMEDD菌株菜油甾醇的产量。In order to increase the yield of campesterol in Yarrowia lipolytica PMEDD, the fermentation medium composition was optimized. The influences of carbon source type, nitrogen source type, carbon source mass concentration, nitrogen source mass concentration, and KH2PO4mass concentration on the yield of campesterol were explored by single factor experiment. Then the fermentation medium composition was optimized by response surface methodology. The results showed that the optimal medium composition was obained as follows: glucose 3.17 g/100 mL, peptone 1.44 g/100 mL, KH2PO40.65 g/100 mL, Uracil 0.1 g/100 mL, YNB 0.42 g/100 mL, MgSO4·7H2O 0.15 g/100 mL. Under these conditions, the maximal campesterol production of 16.49 μg/mL was obtained, which was 2.10 times of the initial fermentation medium. The optimized medium can effectively increase the yield of campesterol in Yarrowia lipolytica PMEDD.

关 键 词：解脂亚罗酵母 菜油甾醇 响应面法 培养基优化 

分 类 号：TQ645.98[化学工程—精细化工] TQ920.1[轻工技术与工程—发酵工程]