不同蛋白原料对绵羊瘤胃体外发酵参数和氨基酸含量的影响  

Effect of different protein sources on in vitro rumen fermentation parameters and amino acid content in sheep

作　　者：杨彩虹 田星哲 李金辉 严慧 段春辉[1] 张英杰[1] 纪守坤 刘月琴[1] YANG Cai-hong;TIAN Xing-zhe;LI Jin-hui;YAN Hui;DUAN Chun-hui;ZHANG Ying-jie;JI Shou-kun;LIU Yue-qin

机构地区：[1]河北农业大学动物科技学院,河北保定071000

出　　处：《饲料研究》2023年第4期5-11,共7页Feed Research

基　　金：河北省重点研发(农业高质量发展关键共性技术攻关专项)项目(项目编号:19226627D);河北省自然科学基金青年基金项目(项目编号:C2020204181);国家重点研发计划项目(项目编号:2018YFD0502100);国家肉羊产业技术体系(项目编号:CARS-38)。

摘　　要：试验旨在研究不同蛋白原料对绵羊瘤胃体外发酵参数和氨基酸含量的影响。试验采用体外发酵法,以豆粕、棉仁粕、花生粕、菜籽饼、干酒糟及其可溶物(DDGS)和玉米胚芽饼为发酵底物,测定产气量、消化率、瘤胃液发酵参数、挥发性脂肪酸(VFA)及氨基酸含量。结果显示,杂粕各组的48 h产气量(GP)显著低于豆粕组(P<0.05)。玉米胚芽饼组的粗蛋白(CP)降解率显著高于豆粕组(P<0.05),棉仁粕、花生粕、菜粕、DDGS组显著低于豆粕组(P<0.05)。棉仁粕、菜粕组的氨态氮(NH_(3)-N)含量显著高于豆粕组(P<0.05),棉仁粕组乙酸、丙酸、丁酸、异丁酸、异戊酸、总挥发性脂肪酸含量和乙丙比显著高于豆粕组(P<0.05),菜粕组的乙丙比显著高于豆粕组(P<0.05),玉米胚芽饼组的戊酸含量显著高于豆粕组(P<0.05)。花生粕、菜粕、DDGS组总脂肪酸含量显著高于豆粕组(P<0.05),棉仁粕组亮氨酸(Leu)、赖氨酸(Lys)、蛋氨酸(Met)、精氨酸(Arg)、谷氨酸(Glu)含量显著高于豆粕组(P<0.05),花生粕、菜粕、DDGS组的苏氨酸(Thr)、Leu、Met、组氨酸(His)、Arg、异亮氨酸(Ile)、色氨酸(Trp)、丝氨酸(Ser)、丙氨酸(Ala)、脯氨酸(Pro)、天门冬氨酸(Asp)、Glu含量显著高于豆粕组(P<0.05),玉米胚芽饼组的Thr、Arg、酪氨酸(Tyr)、Ser、Pro、Glu含量显著高于豆粕组(P<0.05)。研究表明,与豆粕相似性由高到低分别为玉米胚芽饼、棉仁粕、花生粕、菜粕、DDGS,养殖生产中,可以根据实际情况选择以上蛋白原料适当替代豆粕。The experiment was to study the effect of different protein raw materials on rumen fermentation parameters and amino acid content in vitro of sheep.The experiment used in vitro fermentation method,using soybean meal,cottonseed meal,peanut meal,rapeseed cake,DDGS and corn germ cake as fermentation substrate,the gas production,digestibility,rumen fermentation parameters,VFA concentration and amino acid content were determined.The results showed that the GP48 h of the miscellaneous meal group was significantly lower than that of soybean meal group(P<0.05).The CP degradation rate of corn germ cake group was significantly higher than that of soybean meal group(P<0.05),while that of cottonseed meal,peanut meal,rapeseed meal and DDGS group was significantly lower than that of soybean meal group(P<0.05).The concentration of NH_(3)-N in cottonseed meal and rapeseed meal group was significantly higher than that in soybean meal group(P<0.05).The content of acetic acid,propionic acid,butyric acid,isobutyric acid,isovaleric acid,total volatile fatty acid and ethylene-propylene ratio in cottonseed meal group were significantly higher than that in soybean meal group(P<0.05).The ethylene-propylene ratio in rapeseed meal group was significantly higher than that in soybean meal group(P<0.05).The concentration of valeric acid in corn germ meal group was significantly higher than that in soybean meal group(P<0.05).The concentrations of total fatty acids in peanut meal,rapeseed meal and DDGS group were significantly higher than those in soybean meal group(P<0.05),and the concentrations of Leu,Lys,Met,Arg,Glu in cottonseed meal group were significantly higher than those in soybean meal group(P<0.05).The concentrations of Thr,Leu,Met,His,Arg,Ile,Trp,Ser in peanut meal,rapeseed meal and DDGS group were significantly higher than those in soybean meal group(P<0.05).The concentrations of Ala,Pro,Asp and Glu in corn germ cake group were significantly higher than those in the soybean meal group(P<0.05).The concentrations of Thr,Arg,Tyr,Ser,P

关 键 词：绵羊 蛋白饲料 体外发酵 发酵参数 氨基酸 

分 类 号：S826[农业科学—畜牧学]