不老莓多肽粉提高小鼠免疫力及抗氧化功能特性分析  

The Polypeptide Powder of Aronia melanocarpa Improves the Immunity in Mice and Exhibits Anti-oxidant Function

作　　者：丛峰松[1] 李兴华 董舒梅 班田震 朱甜甜 郝善驰 孙西玉[3] 刘延波[3] CONG Fengsong;LI Xinghua;DONG Shumei;BAN Tianzhen;ZHU Tiantian;HAO Shanchi;SUN Xiyu;LIU Yanbo(School of Life Sciences and Biotechnology,Shanghai Jiao Tong University,Shanghai 200240,China;Longevity Berry Life Technology Co.Ltd.,Shanghai 201700,China;College of Food and Biological Engineering,Henan University of Animal Husbandry and Economy,Zhengzhou 450046,China)

机构地区：[1]上海交通大学生命科学技术学院,上海200240 [2]不老莓生命技术(上海)有限公司,上海201700 [3]河南牧业经济学院食品与生物工程学院,河南郑州450046

出　　处：《现代食品科技》2023年第3期1-9,共9页Modern Food Science and Technology

基　　金：河南省重点研发与推广专项(科技攻关)项目(202102110130;192102110075);河南省重大科技专项(181100211400);河南牧业经济学院重点学科项目(C3060020)。

摘　　要：该研究以不老莓多肽粉为研究对象,研究其体外抗氧化功能以及对小鼠免疫功能的影响。通过测定小鼠体内脾淋巴细胞转化、迟发型变态反应、血清溶血素等指标,结果发现高剂量组与阴性对照组相比,可以明显提高小鼠的迟发型变态反应(P=0.009)、抗体生成能力(P=0.046)、血清溶血素水平(P=0.040)、碳廓清能力(P=0.001)和NK细胞活性(P=0.018);中剂量组对小鼠脾淋巴细胞增殖能力有显著增强作用(P=0.038);低剂量组具有增强小鼠碳廓清能力(P=0.042);其次通过对DPPH自由基测定、羟自由基和超氧阴离子自由基试剂盒的分析,结果显示半清除浓度IC50分别为0.07、13.91、10.00 mg/mL,总抗氧化能力为72.76 U/mL,且DPPH自由基、羟自由基、超氧阴离子自由基的清除率均与不老莓多肽粉的质量浓度呈量效关系。上述研究结果明确了不老莓多肽粉不仅具有增强小鼠机体免疫功能的作用,而且具有一定的抗氧化功能。In this study,the polypeptide powder of Aronia melanocarpa was taken as the research object for investigating its in vitro antioxidant function and its effect on the immune function of mice.By measuring the transformation of spleen lymphocytes,delayed-type hypersensitivity,serum hemolysin and other indicators of mice,it was found that compared with the negative control group,the high-dose group had significantly increased delayed-type hypersensitivity(P=0.009),antibody-producing ability(P=0.046),serum hemolysin level(P=0.040),carbon clearance ability(P=0.001)and NK cell activity(P=0.018);the medium-dose significantly enhanced the proliferation ability of spleen lymphocytes in mice(P=0.038);the low-dose could enhance the carbon clearance ability in mice(P=0.042);Secondly,through the analyses using the DPPH free radical assay,and hydroxyl radical and superoxide anion radical kits,it was found that the half-scavenging concentrations,IC50 values were 0.07 mg/mL,13.91 mg/mL,and 10.00 mg/mL,respectively,and the total antioxidant capacity was 72.76 U/mL.The scavenging rates for DPPH free radicals,hydroxyl radicals,and superoxide anion free radicals were all dose-dependent with the concentration of berry polypeptide.The research results showed that the polypeptide powder of Aronia melanocarpa not only can enhance the immune function of mice,but also exhibits certain antioxidant function.

关 键 词：不老莓 多肽粉 免疫功能 抗氧化 

分 类 号：TS201.4[轻工技术与工程—食品科学]