南极磷虾油对小鼠力竭运动后骨骼肌质膜修复的影响  

Effect of Antarctic Krill Oil Supplementation on Skeletal Muscle Plasma Membrane Repair in Mice after Exhaustive Exercise

作　　者：杨思梦 贺庆 石丽君[1] 吴迎[1] YANG Simeng;HE Qing;SHI Lijun;WU Ying(School of Sport Science,Beijing Sport University,Beijing 100084,China;Aland Health Holding Ltd.,Shanghai 200120,China)

机构地区：[1]北京体育大学运动人体科学学院,北京100084 [2]艾兰得健康控股有限公司,上海200120

出　　处：《食品科学》2023年第5期112-120,共9页Food Science

基　　金：中央高校基本科研业务费专项资金资助项目(2020054);横向联合项目(20191413,20210056)。

摘　　要：目的:研究补充南极磷虾油(krill oil,KO)对小鼠一次性力竭运动后骨骼肌质膜损伤和功能恢复的影响,探讨补充南极磷虾油促进骨骼肌质膜损伤修复的可能机制。方法:选取8周龄雄性C57小鼠60只,随机分为豆油对照(bean oil control,BOC)组、豆油运动(bean oil exercise,BOE)组、磷虾油对照(krill oil control,KOC)组和磷虾油运动(krill oil exercise,KOE)组,KO组连续灌胃4周200 mg/(kg m_(b)·d)磷虾油,豆油组进行等体积豆油灌胃。干预4周后,运动组进行一次性力竭跑台运动,每组6只小鼠在力竭运动后即刻(0 h)、2、6、24、48、72 h进行抓力测试,其余小鼠于运动后2 h取材。比色法检测小鼠血清肌酸激酶(creatine kinase,CK)、乳酸脱氢酶(lactate dehydrogenase,LDH)活力;冰冻切片并采用伊文氏蓝(Evan’s blue dye,EBD)染色法检测小鼠股四头肌质膜完整性;液相色谱-质谱法检测小鼠股四头肌质膜脂肪酸成分;荧光偏振法检测小鼠股四头肌质膜荧光偏振度、质膜微黏度和质膜脂质流动性。结果:1)磷虾油和豆油干预期间,KO组小鼠体质量在第3、4周显著低于豆油组(P<0.05);2)KOE组小鼠力竭运动时间极显著长于BOE组(P<0.01);KOE组小鼠力竭运动后2、6、24 h和48 h四肢抓力显著高于BOE组(P<0.05);3)KOE组血清CK活力极显著低于BOE组(P<0.01);LDH活力显著低于BOE组(P<0.05);4)KOE组骨骼肌EBD阳性肌纤维面积显著小于BOE组(P<0.05);5)BOE组较BOC组骨骼肌质膜不饱和脂肪酸C_(18:3)质量浓度显著降低(P<0.05);KOE组较KOC组骨骼肌质膜饱和脂肪酸C_(9:0)、C_(10:0)、C_(12:0)、C_(18:0)、C_(20:0)和不饱和脂肪酸C_(20:2)质量浓度极显著或显著降低(P<0.01、P<0.05);KOE组骨骼肌质膜不饱和脂肪酸C_(22:5)、C_(22:6)和C_(24:5)质量浓度显著高于BOE组(P<0.05);6)BOE组较BOC组骨骼肌质膜脂质流动性显著降低(P<0.05),而KOE组骨骼肌质膜脂质流动性极显著高于BOE组(P<0.01)。结论:经4周南极磷虾油补充�Objective:To investigate the effects of supplementation of Antarctic krill oil(KO)on muscle plasma membrane injury and functional recovery in mice after exhaustive exercise,and to explore the possible mechanism by which KO supplementation promotes muscle plasma membrane injury repair.Methods:Sixty male C57 mice aged eight weeks were randomly divided into a soybean oil control group(BOC),a soybean oil plus exercise group(BOE),a krill oil control group(KOC)and a krill oil plus exercise group(KOE).The mice in the KO groups were intragastrically adminsitered with 200 mg/(kg m_(b)·d)of KO for four weeks,and those in the BO groups were given an equal amount of soybean oil.Thereafter,the mice in the exercise groups were subjected to one-time exhaustive treadmill exercise.Six mice in each group were tested for grasping force immediately(0 h),6,24,48 and 72 h after exhaustive exercise,and the rest were sacrificed 2 h after exhaustive exercise.The activities of serum creatine kinase(CK)and lactate dehydrogenase(LDH)were measured by colorimetric assays.The integrity of mouse quadriceps muscle plasma membrane was detected by Evan’s blue dye(EBD)staining.The fatty acid composition of mouse quadriceps muscle plasma membrane was detected by liquid chromatography-mass spectrometry(LC-MS).The fluorescence polarization degree,microviscosity and fluidity of mouse quadriceps muscle plasma membrane were measured by fluorescence polarization method.Result:1)After four and three weeks of intervention,the body mass of mice in the KO groups was significantly lower than that of mice in the BO groups(P<0.05).2)The exhaustive exercise time of mice in the KOE group was significantly longer than that in the BOE group(P<0.01),and the grasping force of limbs in mice in the KOE group was significantly higher than that in BOE group at 2,6,24 and 48 h after exhaustive exercise(P<0.05).3)The serum activity of CK and LDH in mice in the KOE group was significantly lower than that in the BOE group(P<0.01 and 0.05,respectively).4)The area of EBD pos

关 键 词：南极磷虾油 力竭运动 质膜脂质流动性 质膜成分 骨骼肌质膜修复 

分 类 号：R151[医药卫生—营养与食品卫生学]