乙虫腈对意大利蜜蜂工蜂幼虫核酸甲基化的影响  

Effects of Ethiprole on Nuclear Acid Methylation of Apis mellifera ligustica Worker Larvae

作　　者：樊曼 齐素贞 王妙[2] 黄少康 Fan Man;Qi Suzhen;Wang Miao;Huang Shaokang(College of Animal Sciences(College of Bee Science),Fujian Agriculture and Forestry University,Fuzhou 350002,China;Institute of Apicultural Research,Chinese Academy of Agricultural Sciences,Beijing 100093,China;Honey Bee Biology Observation Station of Ministry of Agriculture and Rural Affairs(Fujian),Fuzhou 350002,China)

机构地区：[1]福建农林大学动物科学学院(蜂学学院),福州350002 [2]中国农业科学院蜜蜂研究所,北京100093 [3]农业农村部(福建)蜜蜂生物学观测站,福州350002

出　　处：《生态毒理学报》2023年第1期68-77,共10页Asian Journal of Ecotoxicology

基　　金：中国农业科学院基本科研业务费专项(125161029000160013);福建农林大学动物科学学院(蜂学学院)团队创新基金(13290001006);福州市科技项目(KH190025A,KH190316)。

摘　　要：蜜蜂是重要的环境污染指示生物。作为氟虫腈的替代药剂,乙虫腈对蜜蜂仍有高风险。本文首次基于DNA和RNA甲基化角度评估了亚致死剂量(10^(-5)、10^(-4)、10^(-3)和10^(-2)mg·L^(-1))乙虫腈重复暴露对意大利蜜蜂工蜂幼虫生长发育的影响。结果显示,乙虫腈持续暴露引起了DNA 5mC甲基化水平显著下降和DNA甲基化酶基因Dnmt3显著下调;与DNA相比,RNA(总RNA和mRNA)甲基化所受影响更为显著,各暴露浓度下,总RNA m^(5)C、m^(3)C甲基化和mRNA m^(6)A、m^(3)C甲基化水平均显著提高,RNA m^(6)A去甲基化酶基因ALKBH1均显著下调(P<0.01),m^(5)C甲基化酶基因NSUN4的表达均显著上调(P<0.01)。综上,甲基化酶基因Dnmt3、ALKBH1和NSUN4可作为乙虫腈暴露风险评估的潜在标志物。本研究为杀虫剂的风险预警及评估提供了新的科学视角和技术手段。Honeybee is an important bioindicator for environmental pollutants.As a substitute for fipronil,ethiprole is still a high risk to honeybee.Herein,we evaluated the effects of ethiprole at sublethal concentrations(10^(-5),10^(-4),10^(-3),and 10^(-2)mg·L^(-1))on the DNA and RNA methylation by continuous exposure of Apis mellifera ligustica worker larvae to it.The results showed significant hypomethylation of DNA 5mC and downregulation of Dnmt3 expression level under all test concentrations.Compared to DNA,RNA methylation,including total RNA and mRNA,was more marked.In all of the treatments,the total RNA methylation in m^(5)C and m^(3)C,and mRNA m^(6)A and m^(3)C methylation were significantly upregulated;the expression of ALKBH1 that encoding RNA m^(6)A demethylase was significantly downregulated(P<0.01);while the NSUN4 encoding RNA m^(5)C methyltransferase was signifi-cantly upregulated(P<0.01).In conclusion,Dnmt3,ALKBH1 and NSUN4 are sensitive enough to sublethal doses of ethiprole,suggesting its potential as risk evaluators for ethiprole.This study provides a new perspective and technic for the assessment of pesticide risk.

关 键 词：乙虫腈 意大利蜜蜂 幼虫 亚致死毒性 核酸甲基化 

分 类 号：X171.5[环境科学与工程—环境科学]